System and method for measuring benzo [a] pyrene and metabolite thereof through on-line gel purification

Abstract

The invention relates to a system and a method for measuring benzo [a] pyrene and metabolite thereof through on-line gel purification. The method comprises the following steps of purifying a preparedsample through an efficient gel column after the sample is introduced, cutting part of fraction flowing out of a component to be measured, diluting the cut fraction by using a dilution pump to reducethe solvent strength of the fraction, mixing the fraction by using a mixer, and enriching the fraction by using a solid-phase extraction column; and after the sample is enriched, eluting the enrichedcomponent from the flow direction opposite to the sample enrichment by using a mobile phase of an analysis pump through valve switching, and detecting and measuring the contents of the benzo [a] pyrene and the metabolite thereof through ultra-high performance liquid chromatography column separation and a fluorescence detector. According to the method, efficient purification and solid-phase extraction enrichment of the sample can be realized at the same time, the analysis sensitivity of the method is greatly improved, the pretreatment of the analysis sample is greatly simplified, and the contents of the benzo [a] pyrene and the metabolite thereof in the order of ng / mL in a blood and urine sample can be accurately measured. A sensitive, accurate and reliable novel method is provided for research on the correlation between smoking and health.

Description

technical field [0001] The invention relates to a system for determining benzo[a]pyrene and its metabolites, in particular to a system for on-line gel purification and determination of benzo[a]pyrene and its metabolites, and also relates to a determination method. Background technique [0002] Benzo[a]pyrene (B[a]P) is the most toxic polycyclic aromatic hydrocarbon compound; tumor epidemiological investigations and animal experiments have found that B[a]P has strong carcinogenicity and is closely related to the occurrence of tumors. Correlation, especially highly correlated with the occurrence of human lung cancer. 3-Hydroxybenzo[a]pyrene (3-OHB[a]P) and 1-hydroxybenzo[a]pyrene (1-OHB[a]P) are the most common stable metabolites of B[a]P, It is commonly used as an exposure biomarker of B[a]P to evaluate the exposure level of B[a]P. At the same time, benzo[a]pyrene is an important harmful component in mainstream cigarette smoke, which may enter the human body with cigarette ...

AI Technical Summary

Problems solved by technology

Among these methods, liquid chromatography-mass spectrometry has high sensitivity, but benzo[a]pyrene itself is not easy to ionize, and liquid chromatography-mass spectrometry requires special detectors, so the method is not universal

Liquid chromatography fluorescence detection method has high popularity and low operating cost, but because the contents of B[a]P and its metabolites 1-OHB[a]P and 3-OHB[a]P in smokers are very low, Sample pretreatment needs to use extraction or solid phase extraction enrichment and separation and purification. The process requires multiple concentrations and transfers. The operation is cumbersome and many factors may introduce experimental errors; moreover, the consumption of organic solvents is large, which causes great environmental pollution.

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