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Anti-tumor nano preparation and application thereof in targeted therapy of malignant tumors

A nano-preparation and targeted therapy technology, applied in the field of anti-tumor nano-preparation and its application in targeted therapy of malignant tumors, can solve the problems such as not many, can not achieve the optimal effect, achieve small trauma area, non-toxic Side effects, effect of high photothermal conversion efficiency

Active Publication Date: 2020-05-08
JIANGXI HERBFINE HI TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that photothermal therapy alone cannot achieve the optimal effect, and highly targeting antineoplastic drugs to the tumor site is a major problem to be solved.
At present, there are not many studies on the use of phospholipids to target tumor sites, especially the research and application of targeting tumor sites and combined photothermal therapy, and there are few studies on highly targeted delivery of drugs to tumor sites in combination with chemotherapy. The present invention Made a further contribution in this regard and successfully prepared a new type of high-efficiency anti-tumor drug

Method used

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  • Anti-tumor nano preparation and application thereof in targeted therapy of malignant tumors
  • Anti-tumor nano preparation and application thereof in targeted therapy of malignant tumors
  • Anti-tumor nano preparation and application thereof in targeted therapy of malignant tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation steps of polydopamine nanoparticles and polydopamine nanoparticle core-loaded epidoxorubicin nano preparations are as follows:

[0047] S1: Take 1g of L-cysteine ​​and 6ml of 25% ammonia water and add them to 260ml of 30% ethanol solution, stir and mix evenly, and then add 0.26 mol / 1 dopamine hydrochloride to the mixed solution in a water bath at 35℃ for 10 min. m1, continue to stir and react for 24 hours to obtain PDA-NPS;

[0048] S2: After dialysis in the PDA-NPS dialysis tube obtained in step S1, continue to dilute it by 2 times with water to obtain solution A;

[0049] S3: Add the excipient drug loading agent, water-based binder, and epirubicin (EPI) in a mass ratio of 12:4:1 into 2 ml methanol to fully dissolve them to obtain solution B;

[0050] S4: Take the solution B of step S3 and slowly drop it into the solution A of step S2. Shake while dripping. After mixing well, place the mixture in a 37 ℃ water bath for 30 min; at the end of the water bath, dialy...

Embodiment 2

[0053] The preparation steps of polydopamine nanoparticles and polydopamine nanoparticle core-loaded epidoxorubicin nano preparations are as follows:

[0054] S1: Take 1 g of L-cysteine ​​and 6 ml of 25% ammonia water and add them to 260 ml of 30% ethanol solution, stir and mix evenly, bathe in a dark water bath at 0°C for 5 minutes, and add 0.26 mol / l dopamine hydrochloride to the mixed solution 60 ml, continue to stir and react for 72 h to obtain PDA-NPS;

[0055] S2: After dialysis in the PDA-NPS dialysis tube obtained in step S1, continue to dilute it with water 6 times to obtain solution A;

[0056] S3: Add the excipient drug loading agent, water-based binder, and epirubicin (EPI) in a mass ratio of 6:10:1 into 2 ml of methanol to fully dissolve them to obtain solution B;

[0057] S4: Take the solution B of step S3 and slowly drop it into the solution A of step S2, shake while dripping, and place the mixture in a 45 ℃ water bath for 25 min after mixing thoroughly; at the end of t...

Embodiment 3

[0060] The preparation steps of polydopamine nanoparticles and polydopamine nanoparticle core-loaded epidoxorubicin nano preparations are as follows:

[0061] S1: Take 1 g of L-cysteine ​​and 6 ml of 25% ammonia water and add them to 260 ml of 30% ethanol solution, stir and mix well, bath in a dark water bath at 40°C for 7 minutes, and add 0.26 mol / l dopamine hydrochloride to the mixed solution 80 ml, continue to stir and react for 0.5 h to obtain PDA-NPS;

[0062] S2: After dialysis in the PDA-NPS dialysis tube obtained in step S1, continue to dilute it with water 8 times to obtain solution A;

[0063] S3: Add the excipient drug loading agent, aqueous binder, and epirubicin (EPI) in a mass ratio of 1:2:1 into 2 ml of methanol to fully dissolve them to obtain solution B;

[0064] S4: Take the solution B of step S3 and slowly drop it into the solution A of step S2. Shake while dripping. After mixing thoroughly, place the mixture in a 30 ℃ water bath for 30 min; at the end of the water ...

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Abstract

The invention discloses an anti-tumor nano preparation and application thereof in targeted therapy of malignant tumors. Polydopamine nanoparticles are used as a photothermal conversion material to prepare the anti-tumor nano preparation with high photothermal conversion efficiency, good in-vivo degradability, good biocompatibility and almost no cytotoxicity; meanwhile, the therapeutic effect of killing tumor cells is achieved in combination with photothermal therapy. After the preparation is subjected to tail vein injection into the body of a BALB / C female mouse inoculated with 4T1-luc cells,the photothermal therapy is carried out, the treatment effect is obviously superior to that of conventional treatment, the treatment mode is small in wound area and almost free of toxic or side effects, the administration dosage is reduced, and compared with surgical treatment, the pain is reduced.

Description

Technical field [0001] The invention relates to the research application of chemotherapy combined with photothermal treatment of malignant tumors, in particular to an anti-tumor nano preparation and its application in targeted treatment of malignant tumors. Background technique [0002] In recent years, photothermal therapy has received extensive attention from the academic community and has made good progress. The treatment principle is that the photothermal material absorbs laser light and converts light energy into thermal energy to increase the temperature of the tumor rapidly and make the temperature exceed the tolerance temperature of cancer cells. 50℃ to kill cancer cells. The combined treatment of malignant tumors by multiple methods has significantly increased its effect to a certain extent. The use of photothermal therapy combined with chemotherapy to treat tumors has achieved good results to a large extent, and due to its small side effects and low trauma, it has Good...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/24A61K41/00A61K31/704A61P35/00A61K49/00
CPCA61K9/5123A61K9/5146A61K41/0052A61K31/704A61P35/00A61K49/0008A61K2300/00
Inventor 李翔张婧邹茜
Owner JIANGXI HERBFINE HI TECH
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