Application of low-molecular-weight chondroitin sulfate in preparation of daily chemical product or external preparation

Abstract

The invention relates to a pharmaceutical technology, and in particular relates to an application of low-molecular-weight chondroitin sulfate in preparation of a daily chemical product or external preparation. The molecular weight range of the low-molecular-weight chondroitin sulfate is 500 to 15000, preferably 1000 to 5000; and the content of the low-molecular-weight chondroitin sulfate in the daily chemical product or external preparation is 0.0001-99% by weight, preferably 0.001-5% by weight. The daily chemical product and external preparation provided by the invention can effectively relieve and prevent skin or oral mucosa inflammation, improve related symptoms such as red swelling, itching and pain, promote elimination of human skin acne, promote acne muscle healing, enhance skin cellactivity, resist oxidation or reduce hair loss.

Description

technical field

[0001] The invention relates to pharmaceutical technology, in particular to the use of low molecular weight chondroitin sulfate in preparing daily chemical products and external preparations. Background technique

[0002] Chondroitin Sulfate (CS) is a polysaccharide mainly composed of repeating disaccharide units linked by β(1-3) N-acetylgalactosamine (GalNAc) and glucuronic acid (GlcA). Currently commercialized chondroitin is mainly extracted from the cartilage tissues of pigs, cattle, chickens, ducks, sharks, sturgeons and other animals. Chondroitin sulfate is considered to have various pharmacological activities such as relieving osteoarthritis, lowering blood lipid and blood sugar, anti-cancer metastasis, promoting cell growth, and promoting skin healing. At present, chondroitin sulfate is used in daily cosmetics mainly for hydrating and moisturizing, and it is a common chondroitin sulfate that has not been treated with low molecular weight. It has a rel...

Examples

Embodiment 1

[0045] Embodiment 1. Preparation of low molecular weight chondroitin sulfate

[0046] The steps of preparing low-molecular-weight chondroitin sulfate by catalytic oxidation are as follows: dissolve chondroitin sulfate in water, add copper sulfate (National Medicine Reagent), heat to 50°C, then add hydrogen peroxide solution (National Medicine Reagent), and react at a constant temperature of 50°C After 4h, the heating was stopped. The reaction solution was dialyzed overnight using a dialysis bag with a molecular weight cut-off of 500-15000 (Shanghai Yuanye Biotechnology Co., Ltd.), and the solution was lyophilized to obtain white low molecular weight chondroitin sulfate.

Embodiment 2

[0047] Example 2. Bioactivity identification of low molecular weight chondroitin sulfate

[0048] First, prepare low molecular weight chondroitin sulfate, the steps are as follows: dissolve 0.5g chondroitin sulfate in 100ml water, add 0.032g (0.2mmol) of copper sulfate (Sinopharm Reagent), heat to 50°C, then add 5ml hydrogen peroxide 30% Solution (Sinopharm Reagent). After 4 hours of constant temperature reaction at 50°C, the heating was stopped. The reaction solution was dialyzed overnight using a dialysis bag with a molecular weight cut-off of 1000 (Shanghai Yuanye Biotechnology Co., Ltd.). The solution was lyophilized to obtain 0.2 g of white low molecular weight chondroitin sulfate. Entrust the Institute of Metal Research, Chinese Academy of Sciences to use gel permeation chromatography (Shimadzu LC-20AT) and gel permeation chromatography column (Viscotek, A3000, Col 300*8.0mm) to carry out gel permeation chromatography to the low molecular weight chondroitin sulfate obt...

Embodiment 3

[0089] Embodiment 3. Preparation of Acne Gel

[0090] No. 1 gel prescription: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, low molecular weight chondroitin sulfate (molecular weight 3000) 1%, the balance is deionized water.

[0091] No. 2 gel prescription: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, low molecular weight chondroitin sulfate (molecular weight 800) 1%, and the balance is deionized water.

[0092] No. 3 gel prescription: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, low molecular weight chondroitin sulfate (molecular weight 15000) 1%, the balance is deionized water.

[0093] No. 4 gel composition: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, chondroitin sulfate (molecula...

technical field

[0001] The invention relates to pharmaceutical technology, in particular to the use of low molecular weight chondroitin sulfate in preparing daily chemical products and external preparations. Background technique

[0002] Chondroitin Sulfate (CS) is a polysaccharide mainly composed of repeating disaccharide units linked by β(1-3) N-acetylgalactosamine (GalNAc) and glucuronic acid (GlcA). Currently commercialized chondroitin is mainly extracted from the cartilage tissues of pigs, cattle, chickens, ducks, sharks, sturgeons and other animals. Chondroitin sulfate is considered to have various pharmacological activities such as relieving osteoarthritis, lowering blood lipid and blood sugar, anti-cancer metastasis, promoting cell growth, and promoting skin healing. At present, chondroitin sulfate is used in daily cosmetics mainly for hydrating and moisturizing, and it is a common chondroitin sulfate that has not been treated with low molecular weight. It has a rel...

Examples

Embodiment 1

[0045] Embodiment 1. Preparation of low molecular weight chondroitin sulfate

[0046] The steps of preparing low-molecular-weight chondroitin sulfate by catalytic oxidation are as follows: dissolve chondroitin sulfate in water, add copper sulfate (National Medicine Reagent), heat to 50°C, then add hydrogen peroxide solution (National Medicine Reagent), and react at a constant temperature of 50°C After 4h, the heating was stopped. The reaction solution was dialyzed overnight using a dialysis bag with a molecular weight cut-off of 500-15000 (Shanghai Yuanye Biotechnology Co., Ltd.), and the solution was lyophilized to obtain white low molecular weight chondroitin sulfate.

Embodiment 2

[0047] Example 2. Bioactivity identification of low molecular weight chondroitin sulfate

[0048] First, prepare low molecular weight chondroitin sulfate, the steps are as follows: dissolve 0.5g chondroitin sulfate in 100ml water, add 0.032g (0.2mmol) of copper sulfate (Sinopharm Reagent), heat to 50°C, then add 5ml hydrogen peroxide 30% Solution (Sinopharm Reagent). After 4 hours of constant temperature reaction at 50°C, the heating was stopped. The reaction solution was dialyzed overnight using a dialysis bag with a molecular weight cut-off of 1000 (Shanghai Yuanye Biotechnology Co., Ltd.). The solution was lyophilized to obtain 0.2 g of white low molecular weight chondroitin sulfate. Entrust the Institute of Metal Research, Chinese Academy of Sciences to use gel permeation chromatography (Shimadzu LC-20AT) and gel permeation chromatography column (Viscotek, A3000, Col 300*8.0mm) to carry out gel permeation chromatography to the low molecular weight chondroitin sulfate obt...

Embodiment 3

[0089] Embodiment 3. Preparation of Acne Gel

[0090] No. 1 gel prescription: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, low molecular weight chondroitin sulfate (molecular weight 3000) 1%, the balance is deionized water.

[0091] No. 2 gel prescription: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, low molecular weight chondroitin sulfate (molecular weight 800) 1%, and the balance is deionized water.

[0092] No. 3 gel prescription: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, low molecular weight chondroitin sulfate (molecular weight 15000) 1%, the balance is deionized water.

[0093] No. 4 gel composition: by weight (%), carbomer 0.7%, glycerin 5%, propylene glycol 5%, phenoxyethanol 0.5%, essence 0.05%, citric acid 0.05%, chondroitin sulfate (molecula...

Images