A kind of preparation method of gene chip

A gene chip and gene mutation technology, applied in the field of biomedicine, can solve the problems of poor applicability of gene chips

Active Publication Date: 2021-09-28
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a gene chip covering more gene mutation sites, so as to solve the technical problem of poor applicability of the gene chip in the prior art

Method used

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  • A kind of preparation method of gene chip

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Embodiment Construction

[0040] The flowchart of the gene chip of the present invention can be found in figure 1 , and its specific implementation process is:

[0041] This example is based on the whole genome sequencing data of 2641 Chinese with a sequencing depth of 30 times as the basic data set. Using the whole genome sequencing data as the basic data set, the data of the whole genome can be obtained to avoid gene incompleteness affecting the accuracy of the subsequent gene chips. At the same time, because the whole genome sequencing data is high-resolution data, it is convenient to obtain large and small comprehensive the mutation site. In this embodiment, the whole genome sequencing data of Chinese people is used, so as to utilize the genetic variation sites of Chinese people to prepare a gene chip for Chinese people.

[0042] First, the GATK tool is used to extract gene mutation sites from the basic data set to obtain an original data set. The original data set obtained in this example contai...

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Abstract

The present application discloses a method for preparing a gene chip, comprising: obtaining a first data set containing gene variation sites of a designated population; Data set; obtain a plurality of verification data sets, each of which includes a type of functionally verified gene variation site; determine multiple chip variations based on the candidate data set and the plurality of verification data sets A variety of mutation sites on the chip constitute a gene chip. The gene chip prepared by the invention contains various types of gene variation sites, realizes multiple uses of one chip, and improves the applicability of the chip.

Description

technical field [0001] The application relates to a method for preparing a gene chip, which belongs to the technical field of biomedicine. Background technique [0002] With the successful completion of the Human Genome Project, a new era of human health and life science research has opened. The continuous development of biobanks and the maturation of technology have provided rich sample resources and clinical data support for the research of human diseases, especially major chronic diseases. Using gene chip technology to genotype samples and find specific biomarkers through bioinformatics analysis of cohort genetic data has become a powerful technical means for human beings to overcome a series of complex diseases. The precious value of genotyping data obtained through gene chip technology has been increasingly understood and valued by people. Governments and scientific research institutions around the world have invested a lot of resources to carry out genetic analysis of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/20
CPCG16B20/20
Inventor 徐涛周凯欣王友何顺民陈飞王静
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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