A kind of bulbous flower dormancy release related gene and its application

A flower and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effects of shortening the growth cycle, reducing production costs, important theoretical significance and application potential

Active Publication Date: 2022-03-25
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Little is known about how tulip flower buds and seeds respond to low temperature and the molecular mechanisms that mediate differences in low temperature requirements among cultivars

Method used

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  • A kind of bulbous flower dormancy release related gene and its application
  • A kind of bulbous flower dormancy release related gene and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Tulip dormancy release related gene DRR1 ( D ormancy R release R The acquisition of elated 1):

[0021] 1. Cloning of the DRR1 gene

[0022] In this study, transcriptome analysis of two tulip varieties with significantly different low temperature requirements was carried out, and the differentially expressed gene DRR1 was obtained. Then, according to the results of full-length transcriptome sequencing of the third generation of tulip, primers were designed to clone the DRR1 sequence using cDNA as a template. The sequencing results showed that the CDS region of the DRR1 gene was highly homologous (96.21% homology) to the tulip GBOF-1 gene (GenBank No. AAD56411.1). Then, using the tulip genomic DNA as a template, the genomic sequence of DRR1 was cloned. The sequence analysis showed that DRR1 contains 5 exons and 4 introns. The schematic diagram of the gene structure is shown in figure 1 . The results of protein sequence analysis showed that DRR1 encodes a bHLH transc...

Embodiment 2

[0026] Application of tulip DRR1 gene in the field of plant dormancy release:

[0027] The CDS sequence (SEQ ID NO. 1) of the DRR1 gene was constructed into the pCAMBIA1300 vector, and transformed into Agrobacterium GV3101. The Arabidopsis thaliana genetic transformation was carried out by using the flower soaking method to obtain a number of transgenic plants overexpressing DRR1, which were then detected by the seed germination experiment. Regulation of the tulip DRR1 gene in seed dormancy in Arabidopsis thaliana. The experimental results are as figure 2 In the figure, OX-1 and OX-3 represent two independent DRR1-overexpressing Arabidopsis transgenic lines to illustrate the reproducibility of the results, and WT represents a normal Arabidopsis control without transgenic treatment. Depend on figure 2 It can be seen that the germination rates of OX-1 and OX-3 transgenic plants were good in the case of low temperature vernalization or without low temperature vernalization, e...

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Abstract

The invention belongs to the technical field of plant genetic engineering, and specifically relates to a gene related to dormancy relief of bulbous flowers and its application. The gene is DRR1 Gene, DRR1 The nucleotide sequence of the gene is shown in SEQ ID NO:1. The present invention also relates to DRR1 Cloning of the promoter of the gene. Through the phenotypic analysis of Agrobacterium-mediated stable transformation of Arabidopsis plants, the results showed that the gene has significant functions of replacing low temperature to break plant dormancy, regulating seed germination and flower bud growth. DRR1 The discovery of the gene provides new genetic resources for the cultivation of new varieties of lily bulbs that do not require low temperature treatment or low temperature requirements; at the same time, it provides new genetic resources for ornamental horticulture. The use of this gene greatly shortens the growth cycle of bulbous flowers , which reduces the economic cost of bulb post-harvest treatment and promotion of cultivation.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a related gene for relieving dormancy of bulbous flowers and its application. Background technique [0002] Tulips and other bulbous flowers are important autumn-planting bulbs and play an important role in the international flower trade. Worldwide, the production and consumption of tulips have been developing rapidly. In 2017, my country imported about 350 million tulip bulbs, while the domestic tulip bulbs were less than 5 million. At the same time, due to insufficient post-harvest treatment technology, domestic tulip bulbs have blind flowers or inconsistent flowering periods, and the viewing effect is poor, which further limits the application space of domestic tulip bulbs. In the post-harvest treatment stage of bulbs, temperature is a key factor affecting flower bud differentiation of tulip bulbs. After the tulip bulbs are harvested, the apical meristem i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/113C12N15/82A01H5/10A01H6/20A01H6/56
CPCC07K14/415C12N15/8267
Inventor 王艳平产祝龙赵慧敏
Owner HUAZHONG AGRI UNIV
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