A kind of phosphorylated ovalbumin and its research method for improving the functional properties of daidzein
A technology of ovalbumin and daidzein, which is applied in the field of phosphorylated ovalbumin and the improvement of daidzein functional properties, can solve the problems of low solubility of hydrophobic compounds, high crystallinity, precipitation or crystallization, etc., so as to solve the problems of daidzein Disadvantages of element, improvement of functional properties, effect of inhibiting crystal precipitation
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Embodiment 1
[0055] A preparation method of phosphorylated ovalbumin: S1: 100 mg of ovalbumin is dissolved in water to prepare a solution containing 1% ovalbumin by mass fraction, and 260 μl of 0.47-0.67 mol / l phytic acid solution is added to the solution to adjust After the pH reaches 4, the volume is constant, and the mixed product is obtained after freeze-drying at -50° C. for 24 hours. The mixed product is heated for phosphorylation reaction to obtain phosphorylated ovalbumin.
Embodiment 2
[0057] A research method for improving the functional properties of daidzein by phosphorylated ovalbumin: S1: Dissolving 100 mg of ovalbumin in water and preparing a solution containing 1% ovalbumin by mass fraction, adding 0.47 to 0.67 mol / 260 μl of phytic acid solution in l, adjusted the pH to 4, then constant volume, freeze-dried at -50°C for 24 hours to obtain a mixed product, and the mixed product was subjected to a phosphorylation reaction under dry heating conditions at 90°C for 3 days, and the obtained reaction product was in an ultra- Dialyze in pure water at room temperature and pressure for 2 days, and then freeze-dry the dialysate at -50°C for 24 hours to obtain phosphorylated ovalbumin, which is used as sample 3 (PA-OVA). Set ovalbumin as sample 1 (OVA); then set sample 2. The difference between sample 2 and sample 3 is that sample 1 starts to go through S1 step, but no phytic acid is added, and the obtained product is sample 2 (DH-OVA).
[0058] S2: Preparation ...
Embodiment 3
[0061] A research method for improving the functional properties of daidzein by phosphorylated ovalbumin: take the samples 1, 2, and 3 obtained in Example 2 and dissolve them in ultrapure water, configure them as a solution with a mass fraction of 1%, stir them evenly, and take Add 11.44mg / ml daidzein into 15ml ethanol solution to prepare daidzein with a concentration of 3mM, add 150μl of 0-120μM daidzein solution to the above sample 1, 2, and 3 solutions, and place in a water bath at 25°C and 37°C Incubate in the pot for 1-2 hours.
[0062] The above mixture was measured by fluorescence spectroscopy. (Measurement conditions are: measured at 25°C and 37°C respectively, excitation wavelengths are 280nm and 295nm, emission wavelength range: 250nm-400nm)
[0063] Result analysis:
[0064] The fluorescence intensity of protein decreased with the increase of daidzein concentration, indicating that daidzein quenched the fluorescence of protein.
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