Application of carbon dots as antifreeze materials
A carbon point, cryopreservation technology, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve problems such as unreported antifreeze performance, and achieve the effect of inhibiting the growth of ice crystals
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Embodiment 1
[0034] (1) Preparation of carbon nanodots (G-CDs)
[0035] Dissolve 0.8M glucose in water, stir well to dissolve, then add to the reaction kettle, react at 220°C for 7h, centrifuge the product three times at 12000r / min for 5min, then dialyze and vacuum dry.
[0036] The transmission electron microscope image of the obtained carbon dots is shown in figure 1 As shown, the infrared spectrum is shown in figure 2 As shown, the UV spectrum is shown as image 3 As shown, the X-ray diffraction pattern is shown in Figure 4 shown.
[0037] (2) Antifreeze performance test of nano carbon dots
[0038] Ice crystal growth rate test: Ice crystal morphology changes and ice crystal growth at different degrees of supercooling (ΔT) were observed by a nanoliter osmotic pressure instrument, and the temperature accuracy of the nanoliter osmotic pressure was 0.01°C. The nanoliter osmotic pressure is composed of a cryogenic circulating pump and a temperature controller. Generally, a water-dis...
Embodiment 2
[0041] The preparation of the carbon nano dot solution dispersed in embodiment 2PBS buffer solution (pH=7.4)
[0042] Dissolve 0.8M glucose in water, stir well to dissolve, then add to the reaction kettle, react at 220°C for 7h, centrifuge the product three times at 12000r / min for 5min, then dialyze and vacuum dry. Take 20mg of carbon dots and add them to PBS buffer solution at a concentration of 20mg / mL.
Embodiment 3
[0043] Application experiment of embodiment 3 carbon dots
[0044] The cells used were sheep blood cells. Select 5mL of blood cells, spin at 4000r / min, centrifuge for 5min, centrifuge and wash several times until the suspension is clear. The obtained blood cells were stored in a 4°C refrigerator for later use. Take 50 μL of pre-prepared blood cells, and then add the same volume of carbon dot solution prepared in Example 2, the concentration of carbon dots is 20 mg / mL. Finally, the concentration of carbon dots containing cells was 10 mg / mL. Then store in liquid nitrogen for 8 hours, rewarm the frozen cells in a water bath at 45°C, the damaged blood cells will expose hemoglobin, and then measure the hemoglobin content by ultraviolet light to obtain the cell recovery rate. Figure 8It can be seen that the recovery rate of cryopreserved cells in pure PBS buffer is basically 0%, while the recovery rate of cryopreserved cells in carbon dots dispersed in PBS buffer is basically 60...
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