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Desorption agent for desorbing antigen in aluminum salt adsorption type vaccine and antigen content detection method

A desorption and adsorption technology, applied in the field of detection of desorbent and its antigen content, can solve the problems of protein denaturation, specific protein structure damage, protein structure damage, etc., achieve high accuracy and precision, and maintain immunogen Good desorption effect

Active Publication Date: 2020-06-19
长春生物制品研究所有限责任公司
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  • Abstract
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Problems solved by technology

However, almost all desorbents will damage the protein structure more or less, causing protein denaturation, especially for enveloped viruses, which are easily affected by chemical reagents, pH and temperature, resulting in the destruction of their structure, so that they cannot to test
Studies have shown that using 7.5% sodium bicarbonate solution or 1-5% sodium dodecylsulfonate solution to absorb forest encephalitis and hemorrhagic fever with renal syndrome inactivated vaccines treated with aluminum adjuvant for 2 hours, the The specific protein structure on the surface of the virus antigen causes damage, making the antigen undetectable

Method used

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  • Desorption agent for desorbing antigen in aluminum salt adsorption type vaccine and antigen content detection method
  • Desorption agent for desorbing antigen in aluminum salt adsorption type vaccine and antigen content detection method
  • Desorption agent for desorbing antigen in aluminum salt adsorption type vaccine and antigen content detection method

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Effect test

Embodiment 1

[0023] (1) Fully mix the forest encephalitis virus with an antigen content of 1:32 or the hemorrhagic fever virus with an antigen content of 1:128 and 0.5 mg / ml aluminum hydroxide, and finally add human serum albumin with a final concentration of 0.1% as vaccine stabilizer;

[0024] (2) Enzyme-linked immunosorbent assay was used to detect the antigen content in the mixture obtained in step (1), and the test result was negative, which proved that all antigens were completely adsorbed by aluminum hydroxide (see Table 1 and 2 for details).

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Abstract

The invention discloses a desorption agent for desorbing an antigen in an aluminum salt adsorption type vaccine and an antigen content detection method. The desorption agent is a sodium citrate solution and comprises a nonionic surfactant which stabilizes protein conformation and increases detection specificity. The desorption agent for desorbing an antigen in an aluminum salt adsorption type vaccine can well desorb, meanwhile, a virus antigen structure is not affected or almost not affected, and the immunogenicity of the virus antigen structure is completely kept.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, the invention relates to a desorbing agent for aluminum hydroxide adjuvant in inactivated vaccines of forest encephalitis and hemorrhagic fever and a method for detecting antigen content thereof. Background technique [0002] Forest encephalitis virus antigen and renal syndrome hemorrhagic fever virus antigen are the main active ingredients in forest encephalitis and renal syndrome hemorrhagic fever vaccines, and their effective content is directly related to the protective effect after vaccination. Therefore, the antigen content is forest An important detection index in the quality control of inactivated vaccines for encephalitis and renal syndrome hemorrhagic fever. [0003] Forest encephalitis virus is spherical, with a diameter of 30-40nm. The capsid is icosahedral and has an envelope. It contains hemagglutinin glycoprotein E, which is an important antigenic component of the virus,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N27/447
CPCG01N27/447G01N33/56983
Inventor 李玉华李景良王颖陈娜娜王玲刘晶晶刘岩松孙宏亮杨红育李帅邱野姜崴
Owner 长春生物制品研究所有限责任公司
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