Transplanting method of tilia amurensis tissue culture seedlings

A technique for group and tissue culture seedlings of tilia, applied in the field of plant tissue culture, can solve the problems of low transplanting survival rate, few researches on transplanting tilia, poor adaptability to environmental changes of tilia tissue culture seedlings, etc. The effect of good environmental adaptability, improved survival rate and quality

Active Publication Date: 2020-07-14
JILIN CITY ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are few studies on the transplanting of linden, and the tissue-cultured seedlings o

Method used

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  • Transplanting method of tilia amurensis tissue culture seedlings
  • Transplanting method of tilia amurensis tissue culture seedlings

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0026] Example 1

[0027] A method for transplanting Tilia amurensis tissue culture seedlings includes the following steps:

[0028] (1) Seedling reforming. Before seedling reforming, disinfect and sterilize the involved instruments, roots of tissue culture seedlings and seedling culture medium. The disinfection should be as comprehensive as possible, including the tools and containers used. The roots of tissue culture seedlings should be rinsed with running water before use. After wiping with 75% alcohol, rinse with water again. The working environment adopts 84 disinfection;

[0029] Select well-growing tissue culture seedlings and transfer them to the seedling cultivation medium (500g MS liquid medium, add 1.0mg / l BA1g, activated carbon 0.5g, multivitamin 2g and penicillin 0.5g), and place in a ventilated room temperature environment with an air humidity of 65% , The cultivation time is 3d, and the after cultivation is obtained;

[0030] (2) Cultivation, screening and refining th...

Example Embodiment

[0034] Example 2

[0035] (1) Seedling tempering, disinfection before tempering is the same as in Example 1;

[0036] Select well-growing tissue culture seedlings, transfer them to the seedling cultivation medium (500g MS liquid medium, add 1.0mg / l BA1.5g, activated carbon 0.5g, multivitamin 4g and penicillin 0.75g), and place them in a ventilated room temperature environment with air humidity 80%, the time of refining seedlings is 4d, the seedlings after refining are obtained

[0037] (2) Cultivation, screening and refining the seedlings and transplanting them into the mixed matrix for 3 months, supplementing with MS nutrient solution containing IBA 0.4 mg / l and GA 0.4 mg / l every week until the mixed matrix is ​​poured, and the environmental humidity is 75% ;

[0038] (3) Transplanting. Before transplanting, loosen the soil, water, apply nitrogen, phosphorus and potassium compound fertilizer, trace elements and microbial inoculants, and spray herbicides and insecticides regularly.

...

Example Embodiment

[0041] Example 3

[0042] (1) Seedling tempering, disinfection before tempering is the same as in Example 1;

[0043] Select well-growing tissue culture seedlings, transfer them to the seedling cultivation medium (500g MS liquid medium, add 1.0mg / l BA2g, activated carbon 0.5g, multivitamin 5g and penicillin 1g), and place them in a ventilated room temperature environment with an air humidity of 90%. Refining the seedlings takes 5 days to obtain the seedlings after refining;

[0044] (2) Cultivation, screening and refining the seedlings and transplanting them into the mixed matrix for 2 months, supplementing with MS nutrient solution containing IBA0.5mg / l and GA0.5mg / l every week until the mixed matrix is ​​poured, and the environmental humidity is 80% ;

[0045] (3) Transplanting. Before transplanting, loosen the soil, water, apply nitrogen, phosphorus and potassium compound fertilizer, trace elements and microbial inoculants, and spray herbicides and insecticides regularly.

[0046] ...

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Abstract

The invention discloses a transplanting method of tilia amurensis tissue culture seedlings, and relates to the technical field of plant tissue culture. The method comprises the following steps: hardening seedlings, screening tissue culture seedlings, transferring the tissue culture seedlings into a seedling hardening culture medium, and placing the seedling hardening culture medium in a ventilatedroom-temperature environment with the air humidity of 65-90% for 3-5 days to obtain the hardened seedlings; culturing, screening the hardened seedlings, transplanting the hardened seedlings into a mixed matrix for 2-3 months, and supplementing an MS nutrient solution containing 0.3-0.5 mg/l of IBA and 0.2-0.5 mg/l of GA every week until the mixed matrix is thoroughly watered and environment humidity reaches 70-80%; and transplanting. The screened tissue culture seedlings have good environmental adaptability, the survival rate and quality of the transplanted tissue culture seedlings are improved, the method is remarkable in effect, and the average transplanting survival rate reaches 97%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more specifically relates to a method for transplanting Tilia Tilia tissue culture seedlings. Background technique [0002] Tilia is an arbor, and as the main nectar source plant in China, it has important economic value. [0003] Generally, the cultivation of tissue culture seedlings includes hardening, the purpose of which is to improve the adaptability of tissue culture seedlings to external environmental conditions, improve their photosynthetic ability, promote the robustness of tissue culture seedlings, and finally achieve the purpose of improving the survival rate of tissue culture seedlings transplanted. Domestication should be carried out from environmental factors such as temperature, humidity, light, and presence of bacteria. Within a few days of domestication, the environmental conditions should be similar to those at the time of cultivation; in the later stages of dom...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G24/28A01G24/15A01G24/30A01G24/10A01G17/00
CPCA01G17/005A01H4/00A01H4/001A01G24/10A01G24/15A01G24/28A01G24/30
Inventor 林士杰于建国王梓默朱红波宋子龙勾天兵包广道罗也付世萃于海洋张延明王丽丽杨晶蔡群张忠辉
Owner JILIN CITY ACADEMY OF FORESTRY
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