Application of IGHMBP2 gene as molecular marker for predicting radiation sensitivity
A molecular marker and sensitivity technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as life-threatening, complications, and different radiotoxicity, and achieve the effect of high mutation efficiency and low cost
Active Publication Date: 2020-08-07
CHINA INST FOR RADIATION PROTECTION
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Problems solved by technology
However, patients who received the same dose of radiation therapy showed different radiotoxicity: a few had no obvious toxic effect
Method used
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Embodiment 1
[0013] 1. Preliminary screening of molecular markers for predicting radiation sensitivity using whole-exon capture sequencing technology
[0014] 1) Sample collection, irradiation and chromosome aberration analysis
[0015] Collect peripheral blood from healthy adult males aged 20-30, give 0, 2Gy 60 Co gamma ray irradiation. Chromosomal aberration analysis was performed on samples irradiated with 2Gy gamma rays, and the population was divided into sensitive group, general group and resistant group. Chromosomes were harvested after blood samples were cultured for 52 hours after irradiation, and slides were prepared. 200 metaphases were analyzed per sample.
[0016] 2) Genomic DNA extraction from peripheral blood
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The invention belongs to the technical field of biological detection, and relates to application of an IGHMBP2 gene as a molecular marker for predicting radiation sensitivity. By utilizing the application of the IGHMBP2 gene as the molecular marker for predicting the radiation sensitivity, the radiation sensitivity can be predicted by judging whether the rs1249463 site of the IGHMBP2 gene is highin mutation efficiency and low in cost.
Description
technical field [0001] The invention belongs to the technical field of biological detection, and relates to the use of IGHMBP2 gene as a molecular marker for predicting radiation sensitivity. Background technique [0002] At present, radiation therapy is still the main means of modern cancer treatment, and about 50% of cancer patients need radiation therapy. However, patients who received the same dose of radiation therapy showed different radiotoxicity: a few had no obvious toxic effects, most had clinically mild or moderate toxicities, and a few had severe normal tissue complications , and may even be life-threatening. Therefore, it is necessary to find a molecular marker related to radiosensitivity to predict whether a patient is a radiosensitive (or radioresistant) individual. [0003] The prediction of radiosensitivity is to be able to tailor radiotherapy regimens to individual patients to improve prognosis, so that on the one hand the radiation dose can be reduced to...
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Login to View More IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/156
Inventor 原雅艺董娟聪左雅慧党旭红董豫阳任越张睿凤刘红艳张忠新王婧洁
Owner CHINA INST FOR RADIATION PROTECTION