Chondrogenic differentiation of umbilical cord mesenchymal stem cells and culture medium for chondrogenic differentiation
A technology of differentiation medium and mesenchymal stem cells, applied in the field of chondrogenic differentiation of umbilical cord mesenchymal stem cells and chondrogenic differentiation medium
Active Publication Date: 2020-08-14
山东卓东生物科技有限公司
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[0007] More chondrogenic differentiation inducers of umbi
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The invention discloses chondrogenic differentiation of umbilical cord mesenchymal stem cells and a culture medium for chondrogenic differentiation. According to the invention, proteoglycan and II-type collagen are cartilage specific matrix products; in a control induction group, hUC-MSCs present obvious chondrogenic differentiation under the induction of a basic induction culture medium; and thecontents of the proteoglycan and the II-type collagen in an L-tyrosine derivative 1 induction group and an L-tyrosine derivative 2 induction group are higher than the contents of the proteoglycan andthe II-type collagen in the control induction group, which indicates that L-tyrosine derivatives 1 and 2 in the L-tyrosine derivative 1 and 2 induction groups can further promote chondrogenic differentiation of hUC-MSCs and have a dose effect. Therefore, the L-tyrosine derivatives 1 and 2 have the effect of promoting chondrogenic differentiation of the umbilical cord mesenchymal stem cells in vitro, and are applicable to preparation of the culture medium for promoting chondrogenic differentiation of the umbilical cord mesenchymal stem cells.
Description
technical field [0001] The invention belongs to the field of stem cells, and relates to induction and differentiation of umbilical cord mesenchymal stem cells, in particular to chondrogenic differentiation of umbilical cord mesenchymal stem cells and a chondrogenic differentiation medium. Background technique [0002] Cartilage tissue engineering is an important bioengineering method for the treatment of cartilage damage diseases. In cartilage tissue engineering, the selection of seed cells mainly considers the following points: the cells are easy to obtain and have sufficient sources, and cause little damage to the subjects; low immune rejection; have the potential to induce differentiation and have strong proliferation and differentiation capabilities; Expansion techniques obtain large numbers of passaged cells. [0003] Cartilage repair and regeneration have focused on chondrocytes in the past. Although these cells are easy to obtain and have a low immune prototype, the ...
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Login to View More IPC IPC(8): C12N5/077
CPCC12N5/0655C12N2500/32C12N2506/1369
Inventor 朱阳晨赵玲杨旭
Owner 山东卓东生物科技有限公司