Copper sulfide nano material and preparation method and application thereof
A nanomaterial, copper sulfide technology, applied in biochemical equipment and methods, copper sulfide, nanotechnology and other directions, can solve the problems of anti-degradation, weak migration ability, disordered differentiation, etc., to enhance the function of cartilage differentiation, enhance the Proliferation, the effect of a simple preparation method
Pending Publication Date: 2022-05-27
澳汀斯(广州)生物医药科技有限公司
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Problems solved by technology
However, mesenchymal stem cell therapy still has problems such as disordered differentiation, anti-inflammation, anti-degradation and poor migration ability.
Method used
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Embodiment
[0051] This embodiment provides an engineered mesenchymal stem cell, and the specific preparation method includes the following steps:
[0052] (1) Preparation of copper sulfide nanoparticles: 188 mg of copper nitrate and 363 mg of L-cysteine were dissolved in 200 mL of deionized water, then 744 mg of sodium thiosulfate pentahydrate was dissolved in 10 mL of deionized water, and then slowly Dropwise into the deionized aqueous solution containing copper nitrate and L-cysteine, heated to 90 ° C and stirred for 2 hours after the dropwise addition, cooled to room temperature after the reaction, and then centrifuged at 4000 rpm for 15 minutes to get the precipitate. , using deionized water and absolute ethanol in turn at 4000rpm for 15 minutes, taking the precipitate, repeating the washing for 5 times, and finally taking the precipitate and drying to obtain copper sulfide nanoparticles;
[0053] (2) CuS-NH 2 Preparation of nanoparticles: 72.3 μg of copper sulfide nanoparticles o...
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Abstract
The invention discloses a copper sulfide nano material and a preparation method and application thereof, and belongs to the technical field of material synthesis, the copper sulfide nano material provided by the invention comprises spherical CuS / TGF-beta1-atPC core-shell structure nano particles, the core-shell structure nano particles take copper sulfide nano particles as cores and phosphatidylcholine as shells, and the core-shell structure nano particles are spherical CuS / TGF-beta1-atPC core-shell structure nano particles are spherical CuS / TGF-beta1-atPC core-shell structure nano particles. Wherein the surfaces of the copper sulfide nanoparticles are loaded with amino groups, and the amino groups are grafted with TGF-beta1 plasmid DNA; the copper sulfide nano material can be internalized into the mesenchymal stem cells and continuously play a role in the cells, proliferation and chondrogenic differentiation of the mesenchymal stem cells are promoted, and the added copper sulfide nano particles, TGF-beta1 plasmid DNA and phosphatidylcholine can achieve a synergistic effect, so that the chondrogenic differentiation of the mesenchymal stem cells is promoted. The biological characteristics of proliferation, matrix degradation resistance and the like of the mesenchymal stem cells are further enhanced, and meanwhile, the chondrogenic differentiation function of the mesenchymal stem cells can be enhanced more remarkably.
Description
technical field [0001] The invention belongs to the technical field of material synthesis, and in particular relates to a copper sulfide nanometer material and a preparation method and application thereof. Background technique [0002] The main pathological features of osteoarthritis are cartilage damage, matrix degradation and mild chronic inflammation. Conventional clinical treatment methods mainly include non-steroidal anti-inflammatory drugs, hyaluronic acid, glucocorticoids, etc. The clinical treatment for cartilage damage repair The main methods are microfracture surgery and autologous chondrocyte transplantation. Autologous chondrocyte transplantation is a surgical method to obtain autologous chondrocytes, cultured in vitro, and then implanted back into the knee joint defect by intra-articular injection. Microfractures are drilled with a drill to create a full-thickness defect on the tibial plateau, allowing mesenchymal stem cells in the bone marrow cavity to migrate...
Claims
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Patent Timeline
Login to View More IPC IPC(8): C12N5/0775C01G3/12A61K35/28A61P19/02A61P29/00B82Y5/00B82Y30/00B82Y40/00
CPCC12N5/0662C01G3/12A61K35/28A61P19/02A61P29/00B82Y5/00B82Y30/00B82Y40/00C12N2500/20C01P2002/72C01P2004/04
Inventor 丁长海陆遥蔡宇
Owner 澳汀斯(广州)生物医药科技有限公司