A kind of chemiluminescent immunoassay chip and its application

A chemiluminescence immunoassay and detection chip technology, which is applied in the field of chemiluminescence immunoassay detection chips, can solve problems such as false negative results, low sensitivity, and large errors, and achieve the effects of improving efficiency, avoiding detection errors, and increasing efficiency and detection sensitivity

Active Publication Date: 2021-10-08
深圳市科瑞达生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of large error, high cost, low sensitivity and high concentration of target protein in the detection of target protein (antigen) by existing ELISA, the present invention provides a chemiluminescence immunoassay chip and its application

Method used

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  • A kind of chemiluminescent immunoassay chip and its application
  • A kind of chemiluminescent immunoassay chip and its application
  • A kind of chemiluminescent immunoassay chip and its application

Examples

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Effect test

Embodiment 1

[0067] Using the chemiluminescent immunoassay chip provided by the present invention to simultaneously detect the contents of human chorionic gonadotropin and progesterone in blood samples:

[0068] Since the detected target proteins are human chorionic gonadotropin and progesterone, and the content of human chorionic gonadotropin is very high during pregnancy, three coating partitions can be set on the chemiluminescent immunoassay chip. Wherein the composite material layer on the two coupling substrates coated with the partitioned surface is formed by coupling surface aminated polystyrene material and HCG antibody, and the HCG antibody is HCG antibody treated with dithiothreitol, After blocking with BSA, it is used as a coating partition for detecting human chorionic gonadotropin (that is, coating partition one for detecting human chorionic gonadotropin 31 and coating partition two for detecting human chorionic gonadotropin 32). The remaining composite material layer on the c...

Embodiment 2

[0075] Using the chemiluminescent immunoassay chip provided by the present invention to simultaneously detect the contents of luteinizing hormone, follicle-stimulating hormone and anti-Müllerian hormone in blood samples:

[0076] Since the detected substances are three kinds of luteinizing hormone, follicle-stimulating hormone and anti-Müllerian hormone, three coated partitions can be set on the chemiluminescence immunoassay chip. The composite material layers on the three coupling substrates coated with partitioned surfaces are respectively formed by coupling surface aminated polystyrene materials and corresponding antibodies. The composite material layer on the coupling substrate on the surface of one of the coated partitions is formed by coupling the surface aminated polystyrene material and the LH antibody, as a coated partition 301 for detecting luteinizing hormone; on the other coated partition The composite material layer on the coupling substrate on the surface is form...

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Abstract

The invention relates to the technical field of immunoassay, and specifically discloses a chemiluminescence immunoassay chip and its application. The chemiluminescent immunoassay chip includes a chip body, a sample injection area and a composite reaction area that are arranged in the chip body and communicate with each other; the composite reaction area includes a coating area and a marker storage area that are sequentially connected according to the sample injection direction ; The coating area includes several sequentially connected coating partitions according to the type of the detection substance, and each of the coating partitions is used to detect a specific detection substance. The chemiluminescent immunodetection chip can be applied to the preparation of equipment for detecting hormone protein content in human blood. The chemiluminescence immunoassay chip of the present invention can realize joint detection of multiple detection objects, has high sensitivity, high repeatability and accuracy of detection results, short detection time and saves a lot of detection cost.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a chemiluminescence immunoassay chip and its application. Background technique [0002] Chemiluminescence immunoassay is a non-radioactive immunoassay method developed rapidly in the past 30 years, which belongs to an ultra-high sensitivity micro-determination technique. It combines the chemiluminescence system with the immune reaction. After the chemiluminescence-related substances mark the antibody or antigen to react with the antigen or antibody to be tested, the free chemiluminescence marker is separated and added to other related substances of the chemiluminescence system to generate chemiluminescence. Quantitative or qualitative detection of antigen or antibody. Chemiluminescent immunoassay has unique characteristics of high sensitivity, high specificity, fast, accurate, specific, and automatable, and plays an important role in clinical testing, drug analysis, environm...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N33/76G01N33/74G01N33/543G01N33/535G01N21/76
CPCG01N21/76G01N33/535G01N33/543G01N33/74G01N33/76
Inventor董丽静陆建斌
Owner深圳市科瑞达生物技术有限公司