Chemiluminiscence immunoassay chip and application

A chemiluminescence immunoassay and detection chip technology, applied in the field of chemiluminescence immunoassay chips, can solve the problems of low sensitivity, false negative results, and high cost, and achieve the effects of increasing detection sensitivity, avoiding detection errors, and increasing efficiency.

Active Publication Date: 2020-08-18
深圳市科瑞达生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of large error, high cost, low sensitivity and high concentration of target protein in the detection of target protein (antigen) by existing ELISA, the present invention provides a chemiluminescence immunoassay chip and its application

Method used

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  • Chemiluminiscence immunoassay chip and application
  • Chemiluminiscence immunoassay chip and application
  • Chemiluminiscence immunoassay chip and application

Examples

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Effect test

Embodiment 1

[0067] Using the chemiluminescent immunoassay chip provided by the present invention to simultaneously detect the contents of human chorionic gonadotropin and progesterone in blood samples:

[0068] Since the detected target proteins are human chorionic gonadotropin and progesterone, and the content of human chorionic gonadotropin is very high during pregnancy, three coating partitions can be set on the chemiluminescent immunoassay chip. Wherein the composite material layer on the two coupling substrates coated with the partitioned surface is formed by coupling surface aminated polystyrene material and HCG antibody, and the HCG antibody is HCG antibody treated with dithiothreitol, After blocking with BSA, it is used as a coating partition for detecting human chorionic gonadotropin (that is, coating partition one for detecting human chorionic gonadotropin 31 and coating partition two for detecting human chorionic gonadotropin 32). The remaining composite material layer on the c...

Embodiment 2

[0075] Using the chemiluminescent immunoassay chip provided by the present invention to simultaneously detect the contents of luteinizing hormone, follicle-stimulating hormone and anti-Müllerian hormone in blood samples:

[0076] Since the detected substances are three kinds of luteinizing hormone, follicle-stimulating hormone and anti-Müllerian hormone, three coated partitions can be set on the chemiluminescence immunoassay chip. The composite material layers on the three coupling substrates coated with partitioned surfaces are respectively formed by coupling surface aminated polystyrene materials and corresponding antibodies. The composite material layer on the coupling substrate on the surface of one of the coated partitions is formed by coupling the surface aminated polystyrene material and the LH antibody, as a coated partition 301 for detecting luteinizing hormone; on the other coated partition The composite material layer on the coupling substrate on the surface is form...

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Abstract

The invention relates to the technical field of immunodetection, and particularly discloses a chemiluminiscence immunodetection chip and application. The chemiluminescence immunoassay chip comprises achip main body, and a sample injection area and a composite reaction area which are arranged in the chip main body and are communicated with each other; the composite reaction area comprises a coating area and a marker storage area which are communicated in sequence according to the sample injection direction; the coating area comprises a plurality of coating subareas which are communicated in sequence according to the types of detected objects, and each coating subarea is used for detecting a specific detected object. The chemiluminiscence immunoassay chip can be applied to preparation of equipment for detecting the content of hormone protein in human blood. The chemiluminescence immunoassay chip provided by the invention can realize joint detection of a plurality of detection objects, and has the advantages of high sensitivity, high repeatability and accuracy of detection results, short detection time and saving of a large amount of detection cost.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a chemiluminescence immunoassay chip and its application. Background technique [0002] Chemiluminescence immunoassay is a non-radioactive immunoassay method developed rapidly in the past 30 years, which belongs to an ultra-high sensitivity micro-determination technique. It combines the chemiluminescence system with the immune reaction. After the chemiluminescence-related substances mark the antibody or antigen to react with the antigen or antibody to be tested, the free chemiluminescence marker is separated and added to other related substances of the chemiluminescence system to generate chemiluminescence. Quantitative or qualitative detection of antigen or antibody. Chemiluminescent immunoassay has unique characteristics of high sensitivity, high specificity, fast, accurate, specific, and automatable, and plays an important role in clinical testing, drug analysis, environm...

Claims

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Application Information

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IPC IPC(8): G01N33/76G01N33/74G01N33/543G01N33/535G01N21/76
CPCG01N21/76G01N33/535G01N33/543G01N33/74G01N33/76
Inventor董丽静陆建斌
Owner深圳市科瑞达生物技术有限公司