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A kind of lactone compound and its extraction method and application

An extraction method and compound technology, applied to the field of lactone compound preparation, can solve problems such as no lactone compounds, and achieve the effects of good antibacterial and fungal activity, good cytotoxic activity, and good bacteriostatic activity

Active Publication Date: 2022-02-08
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on lactone compounds in endophytic fungi in feline feces

Method used

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  • A kind of lactone compound and its extraction method and application
  • A kind of lactone compound and its extraction method and application
  • A kind of lactone compound and its extraction method and application

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Embodiment Construction

[0034] Embodiments of the present invention are described in further detail below:

[0035] A kind of lactone compound, the chemical structural formula of this compound:

[0036]

[0037] The extraction method of this compound is as follows:

[0038] (1) Take cat feces endophytic fungus DS37 secondary metabolite (9.2kg) as raw material and use petroleum ether / ethyl acetate / methanol leaching 8 times, combine extract and filter, reclaim mixed solvent, concentrate under reduced pressure, obtain Crude extract 1500g;

[0039] (2) Put the crude product extract obtained in step (1) on a silica gel chromatographic column, and use petroleum ether with a volume gradient of 1:0:0, 0:1:0, 0:1:1, and 0:0:1 respectively / Ethyl acetate / methanol eluent is eluted, obtains A (110g), B (420g), C (700g), D (100g) four components;

[0040] (3) For the B component (420g) extract of step (2), on a silica gel chromatographic column, use different gradients of petroleum ether / ethyl acetate eluen...

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Abstract

The invention discloses a lactone compound and its extraction method and application. The cat feces endophytic fungus DS37 secondary metabolites are extracted with petroleum ether / ethyl acetate / methanol, the extracts are combined and filtered, and the mixed solvent is recovered , concentrated under reduced pressure to obtain the crude product extract; the crude product extract is placed on a silica gel chromatographic column, and is eluted with petroleum ether / ethyl acetate / methanol eluents with different volume gradients to obtain four components A-D; B component extract is on the silica gel chromatographic column, eluted with petroleum ether / ethyl acetate eluent with different volume gradients to obtain six components B1-B6, B4 component continues on the silica gel chromatographic column, with different Volume gradient petroleum ether / ethyl acetate eluent was eluted to obtain thirteen components of B4.1-B4.13, and B4.10 components were separated by hydroxypropyl sephadex column chromatography to obtain the inner Esters. The invention separates the lactone compound from the secondary metabolite of the endophytic fungus DS37 in cat feces, and the compound has good antibacterial activity and antitumor activity.

Description

technical field [0001] The invention relates to the field of preparation of lactone compounds, in particular to a lactone compound and its extraction method and application. Background technique [0002] Decaisnea insignis (Griff.) Hook.f. & Thomson) is also called Decaisnea insignis (Griff.) Hook.f. & Thomson, and is a plant of the genus Decaisnea insignis (Lardiya balaeeae). Clumping deciduous erect shrubs, widely distributed in southwestern and central China. Akebia plant cat feces is a traditional folk Chinese herbal medicine in my country. It tastes pungent, sweet, and flat in nature. The root and fruit are sweet and cool. So far, research on cat feces has mainly focused on its phytochemical components, while relatively little research has been done on its endophytic fungi. Endophytic fungi refer to fungi that live in plants during at least one stage of their growth without causing significant adverse effects on plant tissues. According to reports, the main types of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/16A01N43/16A01P1/00A01P3/00A61K31/37A61P35/00
CPCC07D311/16A01N43/16A61P35/00
Inventor 杨秀芳惠昱昱王宁宁马养民汪小钢姬海刚
Owner SHAANXI UNIV OF SCI & TECH
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