Method for detecting mating opportunity of cow

A timing and detection line technology, applied in the field of detection, can solve the problems of large size, need for multiple insemination, difficult to accurately grasp, infertility, etc., to achieve the effect of saving manpower, increasing the reproduction rate, and improving the success rate of breeding

Pending Publication Date: 2020-08-21
昆明天沃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually 1-2 times of insemination in one estrus period, because it usually takes several hours for the sperm to reach the ampulla of the fallopian tube and finally have the ability to fertilize, and the survival time in the reproductive tract is longer than the time for the egg to maintain the fertilization ability, so it will pass It is estimated that the first insemination will be performed several hours before ovulation, and the second insemination will be performed before the estimated loss of egg fertilization ability. However, in the

Method used

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  • Method for detecting mating opportunity of cow
  • Method for detecting mating opportunity of cow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The bovine luteinizing hormone LH test paper is prepared by the following method:

[0042] 1) Prepare the components as follows:

[0043] 11) Gold solution: Add 2ml of chloroauric acid solution with a mass concentration of 1%, 2ml of sodium citrate with a mass concentration of 1%, add purified water to 100ml, heat until the solution turns purple, cool to room temperature, and obtain a gold solution ;

[0044] 12) Blocking solution: Dissolve 0.005g bovine serum albumin in 300ul purified water, add 30ul potassium carbonate solution, and mix well to obtain 330ul blocking solution;

[0045] 13) Sample pad treatment solution: completely dissolve 0.605g of tricarboxymethylaminomethane and 1.5g of sodium chloride in 100ml of purified water to obtain a sample pad treatment solution;

[0046] 14) Colloidal gold conjugate diluent: mix 0.605g tricarboxymethylaminomethane, 0.2g polyvinylpyrrolidone, 5g sucrose, 0.9g sodium chloride, 0.5g bovine serum albumin, 3ml sheep serum, 0.1...

Embodiment 2

[0064] The method for detecting the timing of breeding of cattle comprises the following steps:

[0065] 1) Sampling, collect 10ml of urine that the cow urinates 1 hour after the last urination;

[0066] 2) Detection, insert a piece of luteinizing hormone LH detection test paper of Example 1 into the urine of step 1), take it out for 10 seconds, and observe whether the quality control line C and the detection line T in the test paper are obvious after 15 minutes. color:

[0067] 3) The test results are as follows:

[0068] The quality control line C of the test paper has no color, and the test line T has color, indicating that the test result is invalid;

[0069] 4) Repeat steps 1) and 2) again to obtain the following test results:

[0070] Both the quality control line C and the detection line T of the test paper develop color, use the color development on the test paper and the LH color comparison card (such as figure 2 ) for the following comparisons;

[0071] 41) Lut...

Embodiment 3

[0073] The method for detecting the timing of breeding of cattle comprises the following steps:

[0074] 1) Sampling, collecting 8ml of urine that the cow urinates 1 hour after the last urination;

[0075] 2) Detection, insert a luteinizing hormone LH detection test paper of Example 1 into the urine of step 1), take it out for 8 seconds, and observe whether the quality control line C and the detection line T in the test paper are significantly different after 16 minutes. color:

[0076] 3) The test results are as follows:

[0077] The quality control line C of the test paper develops color, but the detection line T does not develop color, which indicates that the LH level of the cow is low. Repeat steps 1) and 2) the next day: both the quality control line C and the detection line T of the test paper develop color , use the color development on the test paper and the LH color card (such as figure 2 ) for the following comparisons;

[0078] Luteinizing hormone LH is 20mIU / ...

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Abstract

The invention provides a method for detecting the mating opportunity of a cow. The method comprises the following steps of: collecting the urine of the cow urinating 1-2 hours later after last time urination; inserting luteinizing hormone LH detection test paper of the cow into the urine in step 1) for 5-10 seconds, and observing whether a quality control line C and a detection line T in the testpaper develop colors or not after 15-18 minutes: the quality control line C does not develop color and the detection line T develops colors, indicating that the detection result is invalid; if the quality control line C develops color and the detection line T does not develop color, indicating that the luteinizing hormone LH level of the cow is low; if both the quality control line C and the detection line T develop colors, performing contrast with an LH colorimetric card; when the LH is less than or equal to 10mIU/ml, indicating that egg cells of the cow are immature and cannot be mated; whenthe LH is 10-25mIU/ml, indicating that the cow enters an ovulation period, repeating the steps 1)-3) every other 4 hours for detection until the LH reaches 45-100mIU/m, indicating that the ovulationtime of the cow is within 5-10 hours, and then performing mating.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting the mating timing of cattle, and belongs to the technical field of animal detection methods. Background technique [0002] At present, in the breeding process of cattle, the timing of mating or insemination of cattle is usually selected during the natural estrus period of cows. In actual production, it is mainly through testing, observing its behavior, checking the vagina and its secretions, rectal examination and touching the ovary, etc. to judge the ovulation time of the cow, and to guide natural breeding or artificial insemination. Usually 1-2 times of insemination in one estrus period, because it usually takes several hours for the sperm to reach the ampulla of the fallopian tube and finally have the ability to fertilize, and the survival time in the reproductive tract is longer than the time for the egg to maintain the fertilization ability, so it will pass It is...

Claims

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Application Information

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IPC IPC(8): G01N33/76G01N33/577
CPCG01N33/76G01N33/577G01N2333/59
Inventor 金义达
Owner 昆明天沃生物科技有限公司
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