Method for detecting mating opportunity of cow
A timing and detection line technology, applied in the field of detection, can solve the problems of large size, need for multiple insemination, difficult to accurately grasp, infertility, etc., to achieve the effect of saving manpower, increasing the reproduction rate, and improving the success rate of breeding
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Embodiment 1
[0041] The bovine luteinizing hormone LH test paper is prepared by the following method:
[0042] 1) Prepare the components as follows:
[0043] 11) Gold solution: Add 2ml of chloroauric acid solution with a mass concentration of 1%, 2ml of sodium citrate with a mass concentration of 1%, add purified water to 100ml, heat until the solution turns purple, cool to room temperature, and obtain a gold solution ;
[0044] 12) Blocking solution: Dissolve 0.005g bovine serum albumin in 300ul purified water, add 30ul potassium carbonate solution, and mix well to obtain 330ul blocking solution;
[0045] 13) Sample pad treatment solution: completely dissolve 0.605g of tricarboxymethylaminomethane and 1.5g of sodium chloride in 100ml of purified water to obtain a sample pad treatment solution;
[0046] 14) Colloidal gold conjugate diluent: mix 0.605g tricarboxymethylaminomethane, 0.2g polyvinylpyrrolidone, 5g sucrose, 0.9g sodium chloride, 0.5g bovine serum albumin, 3ml sheep serum, 0.1...
Embodiment 2
[0064] The method for detecting the timing of breeding of cattle comprises the following steps:
[0065] 1) Sampling, collect 10ml of urine that the cow urinates 1 hour after the last urination;
[0066] 2) Detection, insert a piece of luteinizing hormone LH detection test paper of Example 1 into the urine of step 1), take it out for 10 seconds, and observe whether the quality control line C and the detection line T in the test paper are obvious after 15 minutes. color:
[0067] 3) The test results are as follows:
[0068] The quality control line C of the test paper has no color, and the test line T has color, indicating that the test result is invalid;
[0069] 4) Repeat steps 1) and 2) again to obtain the following test results:
[0070] Both the quality control line C and the detection line T of the test paper develop color, use the color development on the test paper and the LH color comparison card (such as figure 2 ) for the following comparisons;
[0071] 41) Lut...
Embodiment 3
[0073] The method for detecting the timing of breeding of cattle comprises the following steps:
[0074] 1) Sampling, collecting 8ml of urine that the cow urinates 1 hour after the last urination;
[0075] 2) Detection, insert a luteinizing hormone LH detection test paper of Example 1 into the urine of step 1), take it out for 8 seconds, and observe whether the quality control line C and the detection line T in the test paper are significantly different after 16 minutes. color:
[0076] 3) The test results are as follows:
[0077] The quality control line C of the test paper develops color, but the detection line T does not develop color, which indicates that the LH level of the cow is low. Repeat steps 1) and 2) the next day: both the quality control line C and the detection line T of the test paper develop color , use the color development on the test paper and the LH color card (such as figure 2 ) for the following comparisons;
[0078] Luteinizing hormone LH is 20mIU / ...
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Abstract
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