Preparation method of SVF-GEL fat gel

A SVF-GEL, fat technology, applied in biochemical equipment and methods, cell dissociation methods, pharmaceutical formulations, etc., can solve the problems of nodules, hand feeling, necrosis or liquefaction that affect the effect, to ensure uniformity , The effect of improving skin texture and increasing the survival rate

Inactive Publication Date: 2020-09-08
魏露莹
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Problems solved by technology

[0003] When fat (Coleman fat) is filled and transplanted, the adipose tissue is in a free state and loses its blood supply (that is to say, there is no blood to provide them with nutrition for survival and reproduction), it may be necrotic or liquefied, and finally absorbed by the body , the survival rate is not satisfactory, and there may be nodules, which will affect the presentation of the effect or the feel

Method used

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Embodiment Construction

[0024] The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, those of ordinary skill in the art All other embodiments obtained under the premise of no creative work belong to the protection scope of the present invention.

[0025] A kind of SVF-GEL fat glue preparation method, concrete steps are as follows:

[0026] The first step: mechanically emulsify the fat obtained by suction, and let it stand for 5 minutes;

[0027] Step 2: Place the extracted adipose tissue in a high-speed centrifuge for the first centrifugal purification. The centrifuge is controlled at 1000-1800 / min to remove liquid and oil droplets to obtain chyle-shaped nano-fat. Products of adipose stem cells and extracellular matrix;

[0028] Step 3: separate the oil droplets from the liquid, oil dro...

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Abstract

The invention discloses a preparation method of SVF-GEL fat gel. The method comprises the following steps: 1, carrying out mechanical emulsification on fat obtained through suction, and performing standing for 5 min; 2, placing the extracted adipose tissue in a high-speed centrifugal machine, performing first-time centrifugal purification, and controlling the centrifugal machine to be 1000-1800 r / min so as to obtain a product rich in adipose-derived stem cells and extracellular matrixes; 3, independently separating out oil drops from liquid and oil drops obtained through the primary centrifugal purification in the step 2 and obtained chylous nano fat, and performing standing for 5 hours for later use; and 4, mixing the nano-fat subjected to the first centrifugal purification in the step 2with the oil drops in the step 3 in proportion, and performing magnetic stirring to change the nano-fat into floccules. The SVF-GEL fat gel can promote synthesis of skin collagen, achieves the effectsof improving skin quality, resisting wrinkles and promoting skin regeneration, and can completely fill finer wrinkles which cannot be made by traditional Kolman fat.

Description

technical field [0001] The invention relates to the technical field of beauty products, in particular to a method for preparing SVF-GEL fat gel. Background technique [0002] After fat transplantation, the fat is sucked from the part that can be extracted by negative pressure, and after a primary degree of centrifugation-purification-standstill, the fat will be divided into three layers, and the middle layer is granular fat, that is, coleman fat (Coleman fat). Fat), which is currently used for fat acquisition and conventional fat filling for filling and transplantation, in other words, basically using Coleman fat. [0003] When fat (Coleman fat) is filled and transplanted, the adipose tissue is in a free state and loses its blood supply (that is to say, there is no blood to provide them with nutrition for survival and reproduction), it may be necrotic or liquefied, and finally absorbed by the body , the survival rate is not satisfactory, and there may be nodules, which will...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36C12N5/0775
CPCA61L27/3604A61L27/3687A61L27/3691A61L2400/06C12N5/0667C12N2509/00
Inventor 王立惠
Owner 魏露莹
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