A kind of tortoise peptide and its preparation method and application
A sequence and amino acid technology, which is applied in the field of tortoise peptides with sleep-promoting effects and its preparation, can solve the problems of low purity, inability to be artificially synthesized, complex components, etc., achieve stable physical and chemical properties, improve sleep health food, and structure well-defined effects
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Embodiment 1
[0032] 1. Extraction of peptides
[0033] (1) After the stone turtle shell is broken, soak it with 4% volume concentration of acetic acid for 1 hour, then decoct it with water, filter it, collect the decoction solution and concentrate it, and then extract the concentrated solution with petroleum ether to remove fat-soluble impurities, and then The aqueous phase was lyophilized to obtain protein extracts.
[0034] (3) Add the protein extract freeze-dried powder to the NaOH solution with pH 7.5 according to the solid-to-liquid ratio of 1:25 (g / mL), and then add alkaline protease at 1.2% of the weight of the NaOH solution, under the condition of 55°C water bath Enzymolysis was carried out for 6 hours, and then boiled to inactivate the enzyme, filtered, the filtrate was concentrated and freeze-dried to obtain the polypeptide.
[0035] The main components of the final product were determined according to national standards, and the results are shown in Table 1.
[0036] Table 1 T...
Embodiment 2
[0053] Example 2 Animal experiments evaluating the sleep-promoting effect of different polypeptide fragment monomers
[0054] SPF grade Kunming male mice with a body weight of 18±2g were selected. The animal laboratory is SPF clean grade, and the feeding conditions are ambient temperature 25±1°C, humidity 60±10%, light / darkness 12 / 12, free access to food and water. After 3 days of adaptive feeding, the experimental animals were divided into: blank control group, W-G-X-B group, W-G-P-G-B group, W-W-Y-W-R-Hex group, W-R-W-X-H-T-H-N-W group, W-N-A-R-W-P-G-J group, with 10 mice in each group. Under the condition of ensuring normal diet and drinking water, all mice were deprived of food at 8 o'clock every morning, and gavage began at 9 o'clock. The blank group was fed with 0.9% normal saline, and the mice in the four polypeptide segment monomer groups were fed with 50 mg / kg·d corresponding polypeptide monomer sample solution for 14 consecutive days. Two hours after the end of gav...
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