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Method and application of solid-state fermented shredded tobacco stems
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A solid-state fermentation and tobacco stem technology, which is applied in the fields of application, tobacco, and tobacco treatment, can solve the problem of low utilization rate of tobacco stems, and achieve the effect of improving utilization rate and increasing aroma components
Active Publication Date: 2022-04-15
CHINA TOBACCO SICHUAN IND CO LTD
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[0005] The purpose of the present invention is to solve the problem of low utilization rate of tobacco stems, to provide a method for solid-state fermentation of shredded tobacco stems and its application, so as to degrade unfavorable substances in shredded tobacco stems that affect flavor, increase its aroma components, and improve the quality of tobacco stems. Utilization in cigarettes
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Embodiment 1
[0048] (1) Cultivation of slant strains: inoculate Paecilomyces strains on the slant medium (i.e. PDA medium), and cultivate for about 7 days at 30°C until the mycelium covers the slant;
[0049] (2) Liquid seed culture: Inoculate the slant strain into a 250mL Erlenmeyer flask equipped with 80mL seed medium, and cultivate it for 7 days at a shaking table with a rotation speed of 150 rpm and 30°C;
[0050] (3) Paecilomyces fermented broth culture: After the liquid seeds were broken up with glass beads and a magnetic rotor, the liquid seeds were inoculated into the 250mL Erlenmeyer flask of 80mL Paecilomyces fermentation medium according to the volume percentage inoculum of 4%. , cultured at 30°C for 7 days at a shaker speed of 150 rpm;
[0051] (4) Solid-state fermentation culture: after the cultured Paecilomyces fermented liquid is broken up and mixed, take the cultivated Paecilomyces fermented liquid and inoculate it into tobacco stem shreds at an inoculum size of 10% (V / m). ...
Embodiment 2
[0057] Paecilomyces fermented liquid inoculum size is 20% (V / m), and other conditions are the same as embodiment 1.
[0058] Test results: after 2 days, 4 days, and 6 days of fermentation, the changes in components of cut tobacco stems are shown in Table 2. After 6 days of fermentation, the degradation rate of cellulose was 13.75%, the degradation rate of hemicellulose was 14.77%, and the degradation rate of lignin was 11.37%. , the degradation rate of pectin was 15.12%, the increasing rate of reducing sugar content was 34.49%, and the increasing rate of total sugar was 30.99%.
[0059] Table 2:
[0060]
Embodiment 3
[0062] Paecilomyces fermented liquid inoculum size is 30% (V / m), other conditions are the same as embodiment 1.
[0063] Test results: after 2 days, 4 days, and 6 days of fermentation, the changes in components of cut tobacco stems are shown in Table 3. After 6 days of fermentation, the degradation rate of cellulose was 6.62%, the degradation rate of hemicellulose was 18.47%, and the degradation rate of lignin was 18.63%. , the degradation rate of pectin was 14.87%, the increasing rate of reducing sugar content was 13.97%, and the increasing rate of total sugar content was 10.35%.
[0064] table 3:
[0065]
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Abstract
The invention discloses a method for solid-state fermentation of shredded tobacco stems, belonging to the technical field of microbial fermentation. The present invention is based on the solid-state fermentation of tobacco stalk shreds with Paecilomyces fermented liquid, which is mainly realized through the following steps: (1) cultivating strains on inclined planes; (2) cultivating liquid seeds; (3) cultivating Paecilomyces fermented liquid; (4) Solid-state fermentation culture, inoculating the cultured Paecilomyces fermented liquid into a solid-state fermentation medium, and culturing at a temperature of 22-32° C. for 6-10 days. The shredded tobacco stems in the present invention are fermented and biotransformed by the Paecilomyces species, so that the cell wall material of the cut tobacco stems is decomposed and utilized by the Paecilomyces species, and high-efficiency substances such as Paecilomyces polysaccharides and Paecilomyces acids are produced. The biologically active substance reduces the unfavorable factors affecting the internal quality and flavor of the shredded tobacco stem, improves the sensory quality during burning and smoking, and improves the utilization rate of the tobacco stem in cigarettes.
Description
technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a method for solid-state fermentation of shredded tobacco stems and an application thereof. Background technique [0002] As a big tobacco country, my country ranks first in the world in terms of planting area and output of tobacco, and a large amount of tobacco stems are also produced therefrom. At present, the methods for processing tobacco stems mainly include physical blasting, chemical treatment, and biological enzyme methods, which have disadvantages such as poor degradation effect, chemical reagent pollution, and high cost. At present, it is reported that Bacillus pumilus V35 is used to make a bacterial agent for the treatment of cut stems, but it only degrades cellulose and pectin, and its cell wall component lignin is not degraded, so the degradation effect of its cell wall substances is not obvious. At present, the utilization rate of tobacco stems is low,...
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