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A kind of multifunctional oligotrophic cellulose-degrading bacteria compound bacterial agent and its application

A cellulose-degrading bacteria and oligotrophic technology, applied in the application, fungicide, bacteria and other directions, can solve the problems of single function of decomposing agent, crop utilization, eutrophication of water quality and deterioration of soil physical and chemical properties.

Active Publication Date: 2021-07-13
DEZHOU YUANHE AGRI TECH DEVCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing decomposing agents have a single function and do not have high temperature resistance, salt and alkali decomposition, phosphorus and potassium decomposition functions, resulting in incomplete degradation of cellulose, and phosphorus and potassium obtained from degrading cellulose cannot be used by crops
The conventional method of supplementing phosphorus and potassium elements for crops is to apply phosphorus and potassium fertilizers, which directly leads to eutrophication of water quality and deterioration of soil physical and chemical properties

Method used

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  • A kind of multifunctional oligotrophic cellulose-degrading bacteria compound bacterial agent and its application
  • A kind of multifunctional oligotrophic cellulose-degrading bacteria compound bacterial agent and its application
  • A kind of multifunctional oligotrophic cellulose-degrading bacteria compound bacterial agent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Screening and Identification of Example 1 Bacterial Strains Lzh-X14 and Lzh-10

[0030] Strains Lzh-X14 and Lzh-10 are two oligotrophic strains isolated from Datian, Dezhou City, Shandong Province.

[0031] (1) Primary screening of cellulose degrading bacteria

[0032] Use the dilution coating method to apply the dilution to the oligotrophic bacteria screening medium (peptone 1g; yeast extract 0.5g; NaCl 1g; agar 20g, dissolved in deionized water 1000ml. Sterilize at 121°C for 30min.) and the resulting colonies Use a pick loop to pick and inoculate in oligonutrient cellulose Congo red medium (sodium carboxymethylcellulose 1.88g; Congo red 0.25g; MgSO 4 0.025g; K 2 HPO 4 0.05g; mix 20g of agar, dissolve in 1000ml of deionized water. Sterilize at 121°C for 30min. ), select the colony that can produce a transparent circle on this medium and use the three-section line method to purify the bacterial species.

[0033] (2) Screen high temperature and saline-alkali toler...

Embodiment 2

[0044] Antagonism of Bacillus amyloliquefaciens Lzh-X14 and Streptomyces Lzh-10 in embodiment 2 of the present invention

[0045] Bacillus amyloliquefaciens Lzh-X14 and Streptomyces Lzh-10 were inoculated on Gaoshi No. 1 solid medium in a criss-cross pattern, and cultured at 30°C for 24 hours. If there is a bacteriostatic zone, it means that the two strains have antagonistic effect. If the two strains grow cross-growth, the two strains have no antagonistic effect. figure 1 It shows that Bacillus amyloliquefaciens Lzh-X14 and Streptomyces Lzh-10 have no mutual antagonism, and can be used to prepare compound bacterial agents.

[0046] Adopt plate confrontation method to detect the antagonism of Bacillus amyloliquefaciens Lzh-X14 and Streptomyces Lzh-10, inoculate pathogenic bacteria Fusarium moniliforme on the PDA solid medium (200g of peeled potatoes, 20g of sucrose, 20g of agar, 1000mL of distilled water, natural pH), cultured at 30°C for 48 hours and then used for later use....

Embodiment 3

[0047] Example 3 Cellulose Degrading Bacteria Enzyme Activity Determination

[0048] The enzyme activity of cellulase was measured by DNS method, and the strain with higher enzyme activity was selected.

[0049] Experimental method: DNS method to measure cellulase enzyme activity: use the inoculation loop to inoculate the obtained strain into the general liquid medium at 120r / min and cultivate for 12-24h, until the bacterial liquid is turbid, centrifuge at 4000r / min for 10min and take the supernatant as crude Enzyme solution, take 1.0mL enzyme solution and put it into a 15mL test tube, add sodium carboxymethyl cellulose solution at 37°C for 30min, shake evenly, then react in a water bath at 45°C for 30min, add 3.0mL DNS reagent accurately, boil in water for 7min and flush After cooling and oscillating evenly, use a 722 spectrophotometer to compare color at a wavelength of 540nm, record the absorbance against the standard curve, and calculate the enzyme activity. Select the str...

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Abstract

The present invention relates to a multifunctional oligotrophic cellulose degrading bacteria compound bacterial agent and its application, in particular to a compound bacterial agent of multifunctional oligotrophic cellulose degrading bacteria Bacillus amyloliquefaciens Lzh‑X14 and Streptomyces Lzh‑10 and its its application. The compound bacterial agent of the present invention has a strong degrading function on cellulose; it has the effect of antagonizing root rot pathogens and can prevent and control root rot; it has high temperature resistance, salt and alkali resistance, phosphorus and potassium decomposing functions, and can degrade straw more fully. , and make full use of the phosphorus and potassium elements obtained from straw degradation, and can also perform various functions normally in an oligotrophic natural environment; in addition, the present invention can effectively decompose straw and make full use of straw resources.

Description

technical field [0001] The present invention relates to a multifunctional oligotrophic cellulose degrading bacteria compound bacterial agent and its application, in particular to a compound bacterial agent of multifunctional oligotrophic cellulose degrading bacteria Bacillus amyloliquefaciens Lzh-X14 and Streptomyces Lzh-10 and its application. Its application belongs to the fields of agricultural product processing and microbial decomposing agent preparation. Background technique [0002] In recent years, with the rapid development of agriculture, the content of straw in the natural environment is huge, and a large amount of dry straw is produced every year in the world, about 7.4×10 7 Ton. The main component of straw is cellulose, which is not easily degraded under natural conditions. However, only a small amount of straw resources are used, and a large amount of straw is directly burned, which seriously causes waste of resources and environmental pollution. Straw burni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/28A01P3/00C05F11/08C05F17/20C12R1/07C12R1/465
CPCA01N63/22A01N63/28C05F11/08C12N1/20C05F17/20Y02W30/40
Inventor 李正华宋峰冉昆孙晓莉吴娟娟王吉华于家峰曾强成展彬王金涛杜绍国李刻秦张晓飞孙云龙周雯艳董淑敏王铮赵哲
Owner DEZHOU YUANHE AGRI TECH DEVCO