Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of basal medium blended by feed medium and its preparation method and application

A technology of feeding medium and basal medium, which is applied to the application field of the basal medium in cell culture, can solve the problems of long time and heavy workload, and achieves a consistent, low price and novel design idea. Effect

Active Publication Date: 2021-01-29
上海多宁生物科技股份有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are time-consuming and labor-intensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of basal medium blended by feed medium and its preparation method and application
  • A kind of basal medium blended by feed medium and its preparation method and application
  • A kind of basal medium blended by feed medium and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] 1. B-9 basal medium preparation steps:

[0086] (1) Take 800mL ultrapure water, add 100mL feed medium A, 10mL feed medium B, and 10mL B-9 additive, adjust the speed of the stirrer to 800r / min, and stir magnetically for 10min;

[0087] (2) Add 1.8g HEPES to step (1), stir for 10min to make it dissolve completely;

[0088] (3) Add 1.8g P188 to step (2), stir for 10min;

[0089] (4) Add NaHCO in step (3) 3 2.1g, stirred for 10min;

[0090] (5) Add 2g of NaCl in step (4), stir for 10min;

[0091] (6) Utilize 6N HCl to adjust the pH value of the solution in step (5), so that it is in the range of 7.0-7.4;

[0092] (7) Add water to make up to 1L;

[0093] (8) measure the osmotic pressure of the solution that step (7) obtains, its value should be at 280-320mOsm / kg;

[0094] (9) Filtrate with a 0.22 μm filter membrane to obtain the B-9 basal medium.

[0095] 2. Cell culture

[0096] Resuscitate the CHO-K1 engineered cell line from the cell bank, after passage 3 times, t...

Embodiment 2

[0099] 1. B-9 basal medium preparation steps:

[0100] (1) Take 800mL ultrapure water, add 100mL feed medium A, 10mL feed medium B, and 10mL B-9 additive, adjust the speed of the stirrer to 800r / min, and stir magnetically for 10min;

[0101] (2) Add 1.8g HEPES to step (1), stir for 10min to make it dissolve completely;

[0102] (3) Add 1.8g P188 to step (2), stir for 10min;

[0103] (4) Add NaHCO in step (3) 3 2.1g, stirred for 10min;

[0104] (5) Add 2g of NaCl in step (4), stir for 10min;

[0105] (6) Utilize 6N HCl to adjust the pH value of the solution in step (5), so that it is in the range of 7.0-7.4;

[0106] (7) Add water to make up to 1L;

[0107] (8) measure the osmotic pressure of the solution that step (7) obtains, its value should be at 280-320mOsm / kg;

[0108] (9) Filtrate with a 0.22 μm filter membrane to obtain the B-9 basal medium.

[0109] 2. Cell culture

[0110] Resuscitate the CHO-ZN engineered cell line from the cell bank, after passage 3 times, t...

Embodiment 3

[0113] 1. B-9 basal medium preparation steps:

[0114] (1) Take 800mL ultrapure water, add 100mL feed medium A, 10mL feed medium B, and 10mL B-9 additive, adjust the speed of the stirrer to 800r / min, and stir magnetically for 10min;

[0115] (2) Add 1.8g HEPES to step (1), stir for 10min to make it dissolve completely;

[0116] (3) Add 1.8g P188 to step (2), stir for 10min;

[0117] (4) Add NaHCO in step (3) 3 2.1g, stirred for 10min;

[0118] (5) Add 2g of NaCl in step (4), stir for 10min;

[0119] (6) Utilize 6N HCl to adjust the pH value of the solution in step (5), so that it is in the range of 7.0-7.4;

[0120] (7) Add water to make up to 1L;

[0121] (8) measure the osmotic pressure of the solution that step (7) obtains, its value should be at 280-320mOsm / kg;

[0122] (9) Filtrate with a 0.22 μm filter membrane to obtain the B-9 basal medium.

[0123] 2. Cell culture

[0124] Resuscitate the 293F cell line from the cell bank, after passage 3 times, take samples a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of serum-free cell culture medium, and specifically discloses a basal medium blended with a feed medium, including a feed medium A, a feed medium B and additives, the feed medium B The volume consumption is 5-15% of the feed medium A; the feed medium A contains amino acids with a total concentration of 12900-124000mg / L, and inorganic salts and trace elements with a total concentration of 1210-12560mg / mL, A total concentration of 780‑10260 mg / L of vitamins, and a total concentration of 1300‑8020 mg / mL of other components; the feed medium B contains a total concentration of 18000‑180000 mg / L of L‑tryptophan, L‑ Tyrosine, L-cysteine; The additives include sodium pyruvate, ethanolamine, glutathione, spermine, ferric ammonium citrate, and sodium selenite with a total concentration of 0.103-1.251g / L. The basic culture medium of the invention can support the growth and metabolism of mainstream cell lines in the industry and laboratories, has wide-spectrum applicability, low raw material price, simple process operation, and is suitable for mass industrial production.

Description

technical field [0001] The invention relates to the technical field of serum-free cell culture medium, in particular to a basal medium blended with feed medium, a preparation method of the basal medium, and an application of the basal medium in cell culture. Background technique [0002] The history of medicine development in my country has a long history. The ancestors used Chinese herbal medicine to relieve pain and accumulated a wealth of pharmacological knowledge. However, in the face of challenges such as tumors, immune system diseases, and viral infections, small molecule drugs still appear to be powerless. With the development of immunology and molecular biology, the emergence of monoclonal antibody drugs provides a new way to solve these intractable diseases. [0003] Monoclonal antibody drugs are developed based on the principle of specific binding of antigens and antibodies. Monoclonal antibody drugs can neutralize a variety of toxins and make them lose their biol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N5/075
CPCC12N5/0609C12N5/0682C12N5/0686C12N2500/12C12N2500/14C12N2500/16C12N2500/20C12N2500/22C12N2500/24C12N2500/30C12N2500/32C12N2500/36C12N2500/38C12N2500/46C12N2501/999
Inventor 王龙纪明宇王猛
Owner 上海多宁生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com