Application of mogroside IIE in preparation of anti-inflammatory foods, health products or medicines
A technology of mogroside and anti-inflammatory food, which is applied in the field of medicinal natural products, can solve the problems of reducing the expression of inflammatory target proteins iNOS and COX-2, and the low utilization rate of mogroside IIE, achieving the goal of less toxic side effects and broad market prospects Effect
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Embodiment 1
[0036] Example 1 MTT method to detect the effect of mogroside IIE on the survival of RAW264.7 cells
[0037] Purchase mouse macrophages RAW264.7 at the ATCC Model Cell Center in the United States. Cultivate in DMEM medium containing 10% fetal bovine serum (FBS), the incubator environment is constant 37℃ and filled with stable 5% CO 2 gas. After cell resuscitation, cell culture, cell passaging and cell counting, the MTT colorimetric method was used for cytotoxicity determination to detect the effect of mogroside IIE on the survival of RAW264.7 cells.
[0038] The specific measurement steps are as follows:
[0039] Prepare a 96-well plate, collect the cells in good condition, and plate the cells to adjust the density to 4×10 4 / Hole, edge hole medium is filled, after confirming that the cell growth is in good condition, add the concentration gradient of the drug to be tested, set the appropriate gradient, and set the multiple holes, and put it in the incubator for 16-48 hours. Remov...
Embodiment 2
[0041] Example 2 Effect of mogroside IIE on inflammatory protease in RAW 264.7 cells
[0042] Inducible nitric oxide synthase (iNOS) can be produced by a variety of cells. In mammals, iNOS plays an important role in cell damage and host defense. Certain inflammatory mediators such as the bacterial product lipopolysaccharide (LPS) and the cytokine interferon-γ (IFN-γ) are effective inducers of iNOS gene expression. In immune cells, iNOS can induce the production of NO, and NO is a cytotoxic agent that protects the body from various pathogens. Nitric oxide itself is extremely unstable, and it is very difficult to study. Therefore, it is difficult to determine the production of nitric oxide. Enzymes are more convenient and faster.
[0043] Cyclooxygenase 2 (Cyclooxygenase, COX-2) is one of the two subtypes of COX. It is a key enzyme in the metabolism of arachidonic acid. Cyclooxygenase catalyzes the production of arachidonic acid into prostaglandins. Function, inflammation, fever an...
Embodiment 3
[0046] Example 3 Effect of mogroside IIE on prostaglandin E2 (PGE2) in RAW 264.7 cells
[0047] PGE2 is an important lipid mediator, which transmits signals to four different G protein coupled receptors through autocrine and paracrine methods. The role of PGE2 is mainly to transmit signals and participate in gene expression, and induce various pathophysiological reactions, such as brain inflammation, fever, and vasodilation. When the body is subjected to harmful physiological or pathological stimuli, PGE2 will be released in large quantities, and participate in a variety of inflammatory reactions and promote tumor formation by binding to specific receptors on target cells.
[0048] In this example, the anti-inflammatory activity of mogroside IIE was studied by measuring the expression of PGE2. The test results are as follows image 3 As shown: LPS can promote the production of PGE2, but as the concentration of Luo Han Guo IIE increases, the content of PGE2 also decreases gradually....
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