Anti-inflammatory triterpenoid saponin compound, extraction method and application thereof

A technology of triterpenoid saponins and an extraction method, applied in the field of anti-inflammatory triterpenoid saponins and their extraction, can solve the problem that the research on the chemical constituents of Quercus serrata acorns is not thorough enough, and there are no relevant reports on the chemical structure and anti-inflammatory activity. And other issues

Active Publication Date: 2020-10-27
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main active components in Quercus brevifolia acorns are triterpenoid saponins, but the existing researches on the chemical constituents of Quercus brevicina acorns are still not thorough enough. Composition still worthy of further research and development
[0007] The present invention systematically separates the triterpenoid saponins from Quercus brevifolia, and obtains a new class of triterpenoid saponins, whose chemical structure and anti-inflammatory activity have not been reported

Method used

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  • Anti-inflammatory triterpenoid saponin compound, extraction method and application thereof
  • Anti-inflammatory triterpenoid saponin compound, extraction method and application thereof
  • Anti-inflammatory triterpenoid saponin compound, extraction method and application thereof

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Embodiment 1 3

[0088] The extraction of embodiment 1 triterpene saponins

[0089] (1) Take 34.5 kg of dry Quercus brevifolia acorns, divide them into two equal batches of extraction, and extract each batch with 60 liters of 70% ethanol at 60°C for four times under reflux, combine all extracts and concentrate under reduced pressure to extract The volume is 30L;

[0090] (2) The extract in step 1) is extracted with cyclohexane, ethyl acetate and n-butanol in sequence to obtain a 595.29g n-butanol layer;

[0091] (3) Carry out macroporous resin column chromatography with n-butanol layer (500.6g), utilize aqueous methanol gradient elution (H 2 O, 10% aqueous methanol solution, 30% aqueous methanol solution, 50% aqueous methanol solution, 70% aqueous methanol solution, pure methanol; v / v), to obtain 70% methanol aqueous solution eluent (Y) and 100% methanol solution eluent ( Z) valid part;

[0092] (4) Y was subjected to silica gel column chromatography, eluted with chloroform-methanol solutio...

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Abstract

The invention discloses an anti-inflammatory triterpenoid saponin compound, an extraction method and application thereof. The compound is a novel triterpenoid saponin compound, the physicochemical properties and the chemical structure of the compound are determined through identification of physicochemical constants and modern spectroscopy, and powerful reference data are provided for further research, development and utilization of the value of triterpenoid chemical components in quercus serrata acorn. The separation and purification method disclosed by the invention is simple, efficient andmild; meanwhile, pharmacodynamic tests show that the three novel triterpenoid saponin compounds provided by the invention have relatively good in-vitro anti-inflammatory activity and have anti-inflammatory activity on BV-2 cells, thus indicating that the compound, a tautomer thereof and a pharmaceutically acceptable salt thereof have great potential in preparation of novel anti-inflammatory or Alzheimer's disease treatment drugs, and laying a foundation for further development of anti-inflammatory or Alzheimer's disease treatment drugs.

Description

[0001] The patent application of the present invention is a divisional application of the invention patent application with the application date of 2018.06.25, the application number of 201810663783.X, and the invention and creation name of "an anti-inflammatory triterpene saponin compound and its extraction method and application". technical field [0002] The invention relates to the field of medicine, in particular to an anti-inflammatory triterpene saponin compound and its extraction method and application. Background technique [0003] Inflammation is the defense response of living tissue with vascular system to injury factors. Most diseases are accompanied by inflammation. Inflammation will aggravate the occurrence and development of diseases. Some chronic inflammations can lead to tumors. Therefore, the protection against inflammation Control and treatment are of great importance. [0004] Natural medicines, especially those derived from plants, have a variety of chem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07J73/00A61K31/7024A61P29/00A61P25/28A61P1/16A61P11/00A61P25/00A61P1/00A61P9/00A61P15/14
CPCA61P1/00A61P1/16A61P9/00A61P11/00A61P25/00A61P25/28A61P29/00C07J63/008C07J73/008A61P15/14
Inventor 何祥久王宜海雷艳琼黄雨颖
Owner GUANGDONG PHARMA UNIV
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