Human staphylococcus as well as culture method and application thereof
A staphylococcus and culture method technology, applied in the field of microorganisms, can solve problems such as large side effects and unsatisfactory effects of pertussis drugs, achieve high safety, improve respiratory flora, and avoid intestinal diseases.
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[0038] Example 1
[0039] Separation, purification, screening and identification of Staphylococcus hominis WHG2019031202
[0040] 1) Separation and purification of Staphylococcus hominis WHG2019031202
[0041] ①Select 10 healthy children. After the throat is exposed by opening the mouth and making an "ah" sound, use a disposable sterile swab to wipe the secretions from the palatal arch, pharynx and tonsils on both sides with a sensitive and gentle motion;
[0042] ②Smear the swab with throat secretions on the blood agar plate medium (hereinafter referred to as the blood plate) in a zigzag manner, and place it upside down in a 35°C constant temperature incubator for 24 hours;
[0043] ③ Observe and record the growth of the colony after 24 hours;
[0044] ④Using a disposable inoculation loop to pick out individual colonies of different forms on the blood plate, and inoculate them one by one with a three-zone streaking method for pure culture, and put them in a 35°C constant temperature in...
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[0071] Example 2
[0072] Determination of the antibacterial activity of the fermentation broth of Staphylococcus hominis WHG2019031202
[0073] 1) Preparation of sterile filtrate of fermentation broth
[0074] ①Pick a purified bacteria loop of Staphylococcus hominis WHG2019031202 lawn, inoculate it in a test tube containing 10mL broth medium, put it in a 35℃ constant temperature incubator, culture for 24h, and use the bacterial suspension as seed solution .
[0075] Broth medium: peptone 10.0g, beef extract 3.0g, NaCl15.0g, 1L distilled water, pH7.0-7.2.
[0076] ②Add the seed liquid to 5 100mL Erlenmeyer flasks containing 50mL basic fermentation medium according to the proportion of 2‰, 4‰, 6‰, 8‰, 10‰ (V / V), and mark accordingly. 35℃, 150r / min, shaking culture for 72h.
[0077] Basic fermentation medium: glucose 20.0g, tryptone 20.0g, NaCl5.0g, KH 2 PO 4 1.0g, K 2 HPO 4 2.0g, 1L distilled water, pH7.0-7.2.
[0078] ③Draw 1mL of fermentation broth with concentrations of 2‰, 4‰, 6‰,...
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