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A method of improving the effect of acid-base elution in chromatographic decolorization of Lycium barbarum polysaccharides by using ultrasonic waves

A technology of Lycium barbarum polysaccharide and ultrasonic wave is applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., and can solve the problems of low regeneration rate, single hydrochloric acid solution of eluent, unstable color of Lycium barbarum polysaccharide, etc. Stable work and simple operation

Active Publication Date: 2022-03-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It shows that the decolorization effect of Lycium barbarum polysaccharide during static adsorption is not good, and the regeneration rate of the existing resin regeneration method is not high
In order to solve this problem, patent CN110193214 discloses a method for improving the elution effect by using ultrasonic waves, but the eluent used is a single hydrochloric acid solution
Studies have shown that Lycium barbarum polysaccharide pigment is unstable in strong acid or alkali

Method used

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  • A method of improving the effect of acid-base elution in chromatographic decolorization of Lycium barbarum polysaccharides by using ultrasonic waves
  • A method of improving the effect of acid-base elution in chromatographic decolorization of Lycium barbarum polysaccharides by using ultrasonic waves
  • A method of improving the effect of acid-base elution in chromatographic decolorization of Lycium barbarum polysaccharides by using ultrasonic waves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Load and equilibrate the column according to step 1, and flow the prepared Lycium barbarum polysaccharide solution into the column chromatography through a peristaltic pump. After adsorption for 150 min, the adsorption equilibrium is reached. Rinse the resin with distilled water for a period of time, and then turn on the power of the 15 kHz ultrasonic generator. Set it to 81.53W, use the prepared eluent 5% HCl (V / V) and 0.1M NaOH solution, flow into the chromatography column in the order of acid first and then alkali respectively, adjust the speed of the constant flow pump to 4mL / min , start ultrasonic elution and collect the eluate. After the first elution peak, when the absorbance value of the eluent decreases to close to the initial value, the acid washing can be terminated and the alkaline washing stage can be transferred. The final obtained Lycium barbarum polysaccharide decolorization rate was 91.34%. Elution curves such as image 3 As shown, the peak area value...

Embodiment 2

[0030] Pack and equilibrate the column according to step 1. The prepared Lycium barbarum polysaccharide solution was flowed into the column chromatography through the peristaltic pump. After 150min of adsorption, the adsorption equilibrium was reached. The resin was rinsed with distilled water for a period of time, and then the power of the 20kHz ultrasonic generator was turned on. The power was set to 75.72W, and the prepared The eluent 5% HCl (V / V) and 0.1M NaOH solution flowed into the chromatography column in the order of acid first and then alkali respectively, adjust the speed of the constant flow pump to 4mL / min, start ultrasonic elution and collect the washings. Deliquoring. After the first elution peak, when the absorbance value of the eluent decreases to close to the initial value, the acid washing can be terminated and the alkaline washing stage can be transferred. The final obtained Lycium barbarum polysaccharide decolorization rate was 91.37%. The elution curve ...

Embodiment 3

[0032] Pack and equilibrate the column according to step 1. The prepared Lycium barbarum polysaccharide solution was flowed into the column chromatography through the peristaltic pump. After 150min of adsorption, the adsorption equilibrium was reached. The resin was rinsed with distilled water for a period of time, and then the power of the 20kHz ultrasonic generator was turned on. The power was set to 84.39W, and the prepared The eluent 5% HCl (V / V) and 0.1M NaOH solution flowed into the chromatography column in the order of acid first and then alkali respectively, adjust the speed of the constant flow pump to 4mL / min, start ultrasonic elution and collect the washings. Deliquoring. After the first elution peak, when the absorbance value of the eluent decreases to close to the initial value, the acid washing can be terminated and the alkaline washing stage can be transferred. The final obtained Lycium barbarum polysaccharide decolorization rate was 91.31%. Elution curves suc...

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Abstract

The invention discloses a method for improving the effect of acid-base elution in chromatographic decolorization of Lycium barbarum polysaccharide by using ultrasonic waves, and belongs to the fields of Lycium barbarum polysaccharide purification and resin regeneration. The method uses acid first, then alkali, and acid-base combination to elute the pigment adsorbed on the resin, and uses ultrasonic waves to improve the elution effect. It specifically includes the following steps: the crude polysaccharide extract solution of wolfberry is subjected to D280 resin chromatography to obtain the decolorized Lycium barbarum polysaccharide solution; the elution method of the pigment on the D280 resin is acid and alkali sequential elution, and the desorption effect is enhanced by ultrasonic waves. The invention utilizes column chromatography to decolorize the wolfberry polysaccharide, and the decolorization effect is good, and the wolfberry pigment in the resin is eluted by ultrasonic wave and acid-base combination, thereby improving the regeneration effect of the resin.

Description

technical field [0001] The invention belongs to a column chromatography device for eluting resins in the fields of chemical industry, food, pharmacy and the like, in particular to a method for improving the elution effect of acid-base combination in the chromatographic decolorization of wolfberry polysaccharide by using ultrasonic waves Background technique [0002] Lycium barbarum is rich in sugars, among which Lycium barbarum polysaccharide is the main bioactive substance. Modern medicine has proved that Lycium barbarum polysaccharide has the functions of lowering blood pressure, lowering blood lipids, anti-fatigue, anti-oxidation, anti-tumor, anti-aging, regulating immunity, protecting nerves, etc. It can effectively prevent and treat chronic diseases such as diabetes, hyperlipidemia, and immune function deficiency. During the extraction process of Lycium barbarum polysaccharide, there are various impurities such as organic solvent residues, macromolecular glycoproteins, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/42
CPCB01D15/426
Inventor 翟容容马海乐任晓锋周存山陆峰王禹程王一凝
Owner JIANGSU UNIV