Method and separation device for quickly separating sperms from complex samples of legal medical expert

A sample and complex technology, applied in the field of rapid separation of sperm from complicated forensic samples, to achieve the effect of not being fragile, easy to operate, and small in size

Active Publication Date: 2020-11-03
TSINGHUA UNIV
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But microfluidics opens up possibilities for automated preprocessing of forensic samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and separation device for quickly separating sperms from complex samples of legal medical expert
  • Method and separation device for quickly separating sperms from complex samples of legal medical expert

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] The device proposed by the present invention for the rapid separation of sperm from complicated forensic samples has a structure such as figure 1 As shown, it includes a silicon chip 1, a glass chip 2, a drainage tube 4, a piezoelectric ceramic chip 5, a signal amplification circuit 6 and a signal generating module 7; the upper surface of the silicon chip 1 is processed with a plurality of grooves, and there are After the grooved silicon chip 1 and the glass chip 2 are relatively fixed, the groove on the silicon chip 1 and the glass chip 2 form a liquid flow channel, and the drainage tube 4 communicates with the corresponding liquid flow channel through the inverted cone pressure ring 3; The piezoelectric ceramic sheet 5 is relatively fixed to the lower surface of the silicon chip 1; the signal generation module 7 and the signal amplification circuit 6 are connected to the piezoelectric ceramic sheet 5 through signal lines.

[0018] In the above-mentioned device for rap...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of biological testing, in particular to a method and device for quickly separating sperms from a complex sample of a legal medical expert. According to thedevice, through a piezoelectric ceramic piece and a peripheral driving circuit, ultrasonic field force acts on a micro-fluidic chip for rapid sperm separation, and high-purity separation of sperms from the complex mixed sample of the legal medical expert is achieved on the premise that the integrity of various cells in the sample of the legal medical expert is not destroyed. The method provided by the invention has the advantages of rapidness, convenience and the like, can realize high-purity separation of sperms from the complex mixed sample of the legal medical expert on the premise of notdestroying cell integrity, and can remove other suspended impurities in the mixed sample at the same time. The method can be widely applied to sexual invasion cases, sperm samples of criminal suspectscan be rapidly, reliably and accurately obtained from mixed spots, and the sperm samples are used for subsequent experiments so as to obtain biological information of the criminal suspects.

Description

technical field [0001] The invention relates to the technical field of biological testing, in particular to a method and device for rapid separation of sperm from complicated forensic samples. Background technique [0002] Forensic samples in sexual assault cases are often contaminated by the victim's tissue cells and free nucleic acid. Differential extraction methods are commonly used in laboratories to lyse vaginal epithelial cells and sperm cells in sequence, and then analyze the genetic characteristics of the perpetrator. However, this method is complicated to operate and difficult to automate, and it is difficult to completely remove nucleic acid substances contained in female epithelial cells from the obtained sample. The James Landers laboratory of the University of Virginia in the United States developed an ultrasound-based differential extraction method, which captures sperm cells through an ultrasonic resonance field to elute lysed epithelial cells. However, comple...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/00C12N5/076C12N13/00
CPCC12M23/16C12M47/04C12N5/061C12N13/00B01L3/502707B01L3/502761C12N2509/10B01L2400/0439
Inventor 王晗孙凯刘鹏刘冉
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap