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Method and separation device for quickly separating sperms from complex samples of legal medical expert

A sample and complex technology, applied in the field of rapid separation of sperm from complicated forensic samples, to achieve the effect of not being fragile, easy to operate, and small in size

Active Publication Date: 2020-11-03
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But microfluidics opens up possibilities for automated preprocessing of forensic samples

Method used

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  • Method and separation device for quickly separating sperms from complex samples of legal medical expert
  • Method and separation device for quickly separating sperms from complex samples of legal medical expert

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Embodiment Construction

[0017] The device proposed by the present invention for the rapid separation of sperm from complicated forensic samples has a structure such as figure 1 As shown, it includes a silicon chip 1, a glass chip 2, a drainage tube 4, a piezoelectric ceramic chip 5, a signal amplification circuit 6 and a signal generating module 7; the upper surface of the silicon chip 1 is processed with a plurality of grooves, and there are After the grooved silicon chip 1 and the glass chip 2 are relatively fixed, the groove on the silicon chip 1 and the glass chip 2 form a liquid flow channel, and the drainage tube 4 communicates with the corresponding liquid flow channel through the inverted cone pressure ring 3; The piezoelectric ceramic sheet 5 is relatively fixed to the lower surface of the silicon chip 1; the signal generation module 7 and the signal amplification circuit 6 are connected to the piezoelectric ceramic sheet 5 through signal lines.

[0018] In the above-mentioned device for rap...

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Abstract

The invention relates to the technical field of biological testing, in particular to a method and device for quickly separating sperms from a complex sample of a legal medical expert. According to thedevice, through a piezoelectric ceramic piece and a peripheral driving circuit, ultrasonic field force acts on a micro-fluidic chip for rapid sperm separation, and high-purity separation of sperms from the complex mixed sample of the legal medical expert is achieved on the premise that the integrity of various cells in the sample of the legal medical expert is not destroyed. The method provided by the invention has the advantages of rapidness, convenience and the like, can realize high-purity separation of sperms from the complex mixed sample of the legal medical expert on the premise of notdestroying cell integrity, and can remove other suspended impurities in the mixed sample at the same time. The method can be widely applied to sexual invasion cases, sperm samples of criminal suspectscan be rapidly, reliably and accurately obtained from mixed spots, and the sperm samples are used for subsequent experiments so as to obtain biological information of the criminal suspects.

Description

technical field [0001] The invention relates to the technical field of biological testing, in particular to a method and device for rapid separation of sperm from complicated forensic samples. Background technique [0002] Forensic samples in sexual assault cases are often contaminated by the victim's tissue cells and free nucleic acid. Differential extraction methods are commonly used in laboratories to lyse vaginal epithelial cells and sperm cells in sequence, and then analyze the genetic characteristics of the perpetrator. However, this method is complicated to operate and difficult to automate, and it is difficult to completely remove nucleic acid substances contained in female epithelial cells from the obtained sample. The James Landers laboratory of the University of Virginia in the United States developed an ultrasound-based differential extraction method, which captures sperm cells through an ultrasonic resonance field to elute lysed epithelial cells. However, comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/00C12N5/076C12N13/00
CPCC12M23/16C12M47/04C12N5/061C12N13/00B01L3/502707B01L3/502761C12N2509/10B01L2400/0439
Inventor 王晗孙凯刘鹏刘冉
Owner TSINGHUA UNIV
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