Compound microbial agent for resourceful treatment of vegetable residues as well as preparation method and application of compound microbial agent

A technology of vegetable residues and compound bacteria, which is applied in the field of microbial fermentation, can solve the problems of increasing the cost of foreign transportation and landfill treatment, environmental pollution in rural and urban areas, and many types of vegetable residues, and achieves good nitrogen fixation effect and solves the problem of Difficulty in transportation and storage, effect of good environmental effect

Inactive Publication Date: 2020-11-03
榆林沙漠王生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improper disposal of vegetable residues has caused serious pollution to rural and urban environments
Due to the variety, quantity and weight of vegetable residues produced every day, the cost of external transportation and landfill processing is increased

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] A method for preparing a compound microbial agent for recycling vegetable residues according to an embodiment of the present invention comprises the following steps:

[0031] (1) Inoculate Bacillus bh1, Bacillus bh2, Bacillus bh3, and Bacillus cereus on 150ml LB liquid medium respectively, at a temperature of 35-37°C, at a speed of 130-150rpm, and cultivate for 40-50 hours to obtain Bacillus bh1 , Bacillus bh2, Bacillus bh3, Bacillus cereus suspension;

[0032] (2) Inoculate Gordonella on 150ml of beef extract-peptone liquid culture medium, at a temperature of 30° C., at a speed of 100 to 120 rpm, and cultivate for 60 to 70 hours to obtain the Gordonella bacterium liquid;

[0033] (3) Take the same volume of the above five bacterial agents and mix them to obtain a compound bacterial agent. Among them, the above strains can be purchased from Yulin Desert King Biotechnology Co., Ltd.

[0034] In the embodiment of the present invention, the LB medium includes: 10 g of tr...

Embodiment 1

[0042] A method for preparing a compound microbial agent for recycling vegetable residues according to an embodiment of the present invention comprises the following steps:

[0043] LB medium includes: tryptone 10g, yeast extract powder 5g, sodium chloride 10g, deionized water 1000mL.

[0044] The beef extract-peptone medium includes: beef extract 10g, peptone 10g, sodium chloride 5g, deionized water 1000mL.

[0045] (1) Bacillus bh1, Bacillus bh2, Bacillus bh3, and Bacillus cereus were respectively inoculated on 150ml LB liquid medium at a temperature of 35-37°C and a rotation speed of 130rpm, and cultured for 48 hours to obtain an effective viable bacteria content of 5× 10 10 CFU / ml Bacillus bh1, effective live bacteria content is 3×10 11 CFU / ml Bacillus bh2, effective live bacteria content is 4×10 10 CFU / ml Bacillus bh3, effective live bacteria content is 5×10 11 CFU / ml Bacillus cereus suspension;

[0046] (2) Gordonella was inoculated onto 150ml beef extract-peptone l...

Embodiment 2

[0052] A method for preparing a compound bacterial agent for resourceful processing of baby cabbage according to an embodiment of the present invention comprises the following steps:

[0053] LB medium includes: tryptone 10g, yeast extract powder 5g, sodium chloride 10g, deionized water 1000mL.

[0054] Beef extract-peptone medium includes: beef extract 10g, peptone 10g, sodium chloride 5g, deionized water 1000mL.

[0055] (1) Bacillus bh1, Bacillus bh2, Bacillus bh3, and Bacillus cereus were respectively inoculated on 150ml LB liquid medium at a temperature of 35-37°C and a rotation speed of 140rpm, and cultured for 50 hours to obtain an effective viable bacteria content of 6× 10 11 CFU / ml Bacillus bh1, effective live bacteria content is 4×10 10 CFU / ml Bacillus bh2, effective live bacteria content is 5×10 10 CFU / ml Bacillus bh3, effective live bacteria content is 2×10 11 CFU / ml Bacillus cereus suspension;

[0056] (2) Gordonella was inoculated on 150ml beef extract-pepto...

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PUM

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Abstract

The invention discloses a compound microbial agent for resourceful treatment of vegetable residues as well as a preparation method and application of the compound microbial agent. The compound microbial agent is prepared from bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid, bacillus cereus bacterial liquid and gordonia bacterial liquid, wherein the effective viable bacteria contents of the bacillus bh1 bacterial liquid, the bacillus bh2 bacterial liquid, the bacillus bh3 bacterial liquid and the bacillus cereus bacterial liquid are all greater than or equal to 10<10>CFU/ml; and the effective viable bacteria content of the Gordonia bacteria liquid is greater than or equal to 10<9>CFU/ml. The compound microbial agent provided by the invention can increase the composting speed of straw, and increase the maturation degree and the recycling rate of resources.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a compound bacterial agent used for recycling vegetable residues and its preparation method and application. Background technique [0002] At present, a large number of vegetable residues are produced every day in vegetable greenhouses, farmers' markets, etc. Greenhouse growers and vendors mostly pile them up in the fields, let them rot, or throw them into trash cans, and finally transport them to garbage dumps for landfill landfill. Improper disposal of vegetable residues has caused serious pollution to the rural and urban environments. Due to the variety, quantity and weight of vegetable residues produced every day, the cost of external transportation and landfill processing is increased. [0003] In fact, vegetable residues contain a certain amount of carbon, nitrogen, phosphorus, potassium, calcium and other elements, and are rich in organic matte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C05F11/00C12R1/085C12R1/07C12R1/01
CPCC05F11/00C12N1/20C05F17/20Y02W30/40
Inventor 白慧刘永军张至李宇张驰
Owner 榆林沙漠王生物科技有限公司
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