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Eluting machine for purifying proteins

A protein purification and elution machine technology, applied in the field of protein purification, can solve the problems such as the inability to continuously carry out sample loading and washing, the influence of human factors, and the inability to purify proteins, etc. The effect of saving labor time

Active Publication Date: 2020-11-06
新乡医学院三全学院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, protein purification systems are generally only suitable for laboratory process research operations. The degree of automation is low, and it is often impossible to continuously perform sample loading and washing operations. In the purification process, human factors have a great influence and poor repeatability.
At the same time, the traditional operation is to fix the protein purification column on the iron stand, hold the sample tube containing the sample container in one hand, and add the sample with the pipette in the other hand, which has problems such as inconvenient operation, time-consuming and labor-intensive, although In the prior art, protein purification control equipment has been developed, but most of them are single-channel single-control, which cannot be used for large-scale and large-capacity protein purification. Eluent or different types of eluents are used to analyze the elution effect, which requires easy adjustment of the eluent and the addition of multiple sets of elution columns for elution, so as to facilitate research

Method used

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  • Eluting machine for purifying proteins
  • Eluting machine for purifying proteins
  • Eluting machine for purifying proteins

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Embodiment 1

[0019] Such as Figure 1 to Figure 6As shown, a protein purification elution machine according to the present invention adopts a technical solution comprising a body 1, which is characterized in that the inner cavity of the body 1 is divided into a collection layer 101, a purification layer 101, and a purification layer from bottom to top. The reaction layer 102 and the storage layer 103, the storage layer 103 is vertically divided into a first eluent chamber 108, a protein solution chamber 109, a second eluent chamber 110, and an eluent mixing chamber 111 through a partition The top of the body 1 is provided with a first eluent feeding port 105 corresponding to the first eluent chamber 108, and a second eluent is provided at a position corresponding to the second eluent chamber 110. The feed port 107, the first eluent feed port 105 and the second eluent feed port 107 are all provided with a sealing cover plate, the top of the protein liquid cavity 109 is open and a rotating d...

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Abstract

The invention relates to an eluting machine for purifying proteins. The adopted technical scheme is as follows: the eluting machine comprises a machine body, a collecting bucket frame, a purifying column frame, a control system, a first peristaltic pump, a protein preprocessing device, a second peristaltic pump, a transverse moving device, a discharging valve, a hybrid motor, an eluant filling device, a purifying column, a collecting frame, a protein fluid filling pipeline and an eluant filling pipeline. A device capable of acquiring protein raw fluid through crushing protein raw materials andcarrying out filtration is designed and adopts a centrifugal filtration manner and a tangential flow filtration manner, so that the situation that a filtering net is blocked in the filtering processis avoided, and the filtering efficiency is relatively high. Meanwhile, by additionally forming multiple purifying processing channels, preparation and filling of eluants with different concentrationscan be automatically finished according to settings; and by virtue of automatic configuration of the multiple channels, purification and elution of the proteins are more convenient and faster, a lotof labor time can be saved, human errors can be effectively avoided, multiple groups of contrast experiments are convenient to carry out, and elution of the eluent and the proteins are convenient to research.

Description

technical field [0001] The invention belongs to the technical field of protein purification and relates to an eluting machine for protein purification. Background technique [0002] At present, protein purification systems are generally only suitable for laboratory process research operations. The degree of automation is low, and it is often impossible to continuously perform sample loading and washing operations. In the purification process, human factors have a large impact and poor repeatability. At the same time, the traditional operation is to fix the protein purification column on the iron stand, hold the sample tube containing the sample container in one hand, and add the sample with the pipette in the other hand, which has problems such as inconvenient operation, time-consuming and labor-intensive, although In the prior art, protein purification control equipment has been developed, but most of them are single-channel single-control, which cannot be used for large-sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34
CPCC07K1/14C07K1/34
Inventor 胡焕焕姬国杰刘瑞石晓卫关海燕赵巧辉赵枭阳宋小锋王朋博王斌赵萌瑶
Owner 新乡医学院三全学院
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