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Medicine for treating carbon ion radiotherapy-chemotherapy syndromes of esophageal cancer and preparation method thereof

A technology of carbon ions and syndromes, applied in biochemical equipment and methods, drug combinations, pharmaceutical formulations, etc., can solve problems such as difficulty in finding effective single-drug effective monomers, inability to treat esophageal cancer, and high incidence of complications, and achieve Improve the inhibition rate and lethality, good physical strength, and the effect of definite curative effect

Inactive Publication Date: 2020-11-24
罗宏涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, the problems existing in the prior art are: the prior art mainly treats esophageal cancer by surgery, radiotherapy and chemotherapy. , but the incidence of complications is high, and there is no medicine in the field of traditional Chinese medicine that can treat esophageal cancer and alleviate the complications of carbon ion radiotherapy combined with chemotherapy
[0006] Difficulty in solving the above technical problems: there are many kinds of traditional Chinese medicines, and after the combination of prescriptions, the properties of each single drug interact and interfere with each other, and it is difficult to find effective single drugs and effective monomers that really play a role

Method used

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  • Medicine for treating carbon ion radiotherapy-chemotherapy syndromes of esophageal cancer and preparation method thereof
  • Medicine for treating carbon ion radiotherapy-chemotherapy syndromes of esophageal cancer and preparation method thereof
  • Medicine for treating carbon ion radiotherapy-chemotherapy syndromes of esophageal cancer and preparation method thereof

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preparation example Construction

[0039]like figure 1 As shown, the preparation method of the medicine for the treatment of esophageal cancer carbon ion radiotherapy and chemotherapy syndrome provided by the embodiments of the present invention comprises the following steps:

[0040] S101. Weigh the recombinant human superoxide dismutase, Cistanche tuberosa, Myrobalan, Pulsatilla, Fritillaria, Licorice, Coptis Rhizoma, mannitol, trehalose and antioxidants in sequence according to the parts by mass.

[0041] S102, adding a small molecule water solvent into a glass reactor, sequentially adding recombinant human superoxide dismutase, mannitol, trehalose and antioxidants and stirring at a speed of 10-50r / mim to obtain a mixed solution A.

[0042] S103, using 75% ethanol to soak Cistanche tuberosa, repeat extraction twice at a solid-to-liquid ratio of 1:10, each time for 1 hour, and obtain and purify the Cistanche tuberose extract.

[0043] S104, using Pulsatilla Radix and Coptidis Rhizome to prepare the alcohol e...

Embodiment 1

[0064] Esophageal cancer cells Eca-109 and EC9706 were inoculated into 96-orifice plate with the quantity of 4000-6000 cells per well, after the cells adhered to the wall (24h), the medicine of the present invention was diluted into a certain gradient concentration, and each concentration was set at 5 Repeat hole. After 48 hours, use the MTT method to detect cell viability. Add 10 μl of MTT solution to each well and continue to incubate for 4 hours. Carefully suck out the liquid in the wells to avoid contact with crystals in the wells. Add 100 μl of DMSO to each well and shake on a constant speed shaker in the dark. . After the crystals are fully dissolved, read the OD value (wavelength 570nm, reference wavelength 630nm) on a microplate reader, and measure the absorbance A value.

[0065] The calculation formula of growth inhibition rate is: growth inhibition rate (%)=(1-OD)×100%. According to the inhibition rate of each concentration, the dose-response curve can be obtained...

Embodiment 2

[0067] Embodiment 2: Esophageal cancer cell invasion test ( image 3 )

[0068] Before the experiment, Matrigel was taken out from the -20°C refrigerator and placed in a 4°C refrigerator overnight. First, dilute Matrigel to 200ng / ml, add 1640 culture solution at 4°C, spread the diluted liquefied Matrigel on a Transwell polycarbonate membrane, and dry it in a 37°C thermostat for 6 hours to solidify to form a matrix Barrier glue. The prepared Transwell polycarbonate membrane was irradiated with light for 2 hours, and a small amount of sterilized serum-free 1640 medium was added to hydrate the Transwell polycarbonate membrane before use. The Eca-109 and EC9706 cells in the logarithmic phase were washed twice with PBS, digested routinely, centrifuged and the cell culture medium was discarded, the cells were gently rinsed with PBS buffer for 3 times, and the cells were resuspended in BSA-containing medium. in serum-free medium. Adjust the concentration of the cell suspension to...

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Abstract

The invention belongs to the technical field of medicine preparation, and discloses a medicine for treating carbon ion radiotherapy-chemotherapy syndromes of esophageal cancer and a preparation methodthereof. The medicine for treating the carbon ion radiotherapy-chemotherapy syndromes of esophageal cancer is composed of the following components in parts by mass: 30-35 parts of recombinant human superoxide dismutase, 35-40 parts of herba cistanche tubulosae, 25-30 parts of fructus chebulae, 15-20 parts of radix pulsatillae, 5-10 parts of bulbus fritillariae thunbergii, 5-10 parts of liquoriceroots, 5-10 parts of rhizoma coptidis, 10-15 parts of mannitol, 5-8 parts of trehalose and 2-5 parts of an antioxidant. The medicine for treating carbon ion radiotherapy-chemotherapy syndromes of esophageal cancer provided by the invention can reduce harm of chemotherapy and radiotherapy to a human body, and has a good physical recovery effect on the weakness of cancer patients after chemotherapyand radiotherapy. Meanwhile, the medicine can also improve the inhibition rate and lethality of esophageal cancer cells, induce apoptosis of human liver cancer cells and human esophageal cancer cells,and play a role in treating esophageal cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to a medicine and a preparation method for treating esophageal cancer carbon ion radiotherapy and chemotherapy syndrome. Background technique [0002] At present, the commonly used existing technologies in the industry are as follows: esophageal cancer is a common malignant tumor in humans. It is estimated that about 200,000 people die of esophageal cancer every year in the world, and it is the most common disease that is extremely harmful to people's lives and health. One of the malignant tumors. Esophageal cancer is one of the top ten malignant tumors in the world, with an annual increase of 480,000 cases, and its mortality rate ranks sixth among all tumors, with a 5-year survival rate of only 20%. There are about 250,000 new cases every year in my country, accounting for more than half of the total incidence of esophageal cancer. Surgery is the main tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61P35/00A61K36/8966
CPCA61K36/185A61K36/484A61K36/64A61K36/71A61K36/718A61K36/8966A61K38/446A61K47/26A61K2236/331A61K2236/333A61K2236/39A61P35/00C12Y115/01001A61K2300/00
Inventor 罗宏涛张秋宁王小虎刘锐锋刘志强孙世龙杨震邵丽华李征鲁维德冯双午李强马力桢张小奇
Owner 罗宏涛
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