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Tobacco pvy warning gene ntab0691000 and its application
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A gene and tobacco technology, applied in the field of tobacco potato virus disease early warning gene and application patent application matters, can solve the problems of low sensitivity, inability to prevent and control diseases, expensive equipment, etc., and achieve high sensitivity, good application and scientific research value, response fast effect
Active Publication Date: 2022-06-03
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology
Among them, the biological determination method is often unable to be applied in actual control due to the large workload and the symptom response of tobacco plants is often affected by soil, environment and climatic conditions.
Serological determination (ELISA method) is relatively simple to operate, but it is prone to false positives due to problems such as cross-reaction
Although electron microscope technology can directly observe the size and shape of viruses, the equipment is expensive, the technical requirements are high, and the sensitivity is low, so it cannot be practically used for disease control
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Embodiment 1
[0050] In this example, a brief introduction to the screening process of the tobacco potato virus disease early warning gene obtained in the present application is as follows.
[0051] In the previous research process of the tobacco genome, based on the expression profiling results of the Affymetrix tobacco whole genome chip, combined with the significance analysis and MeV variance analysis of the normalized data, P≤0.01 was selected, and the expression value of the treatment group and the control group changed. ≥2 times (after lg2) was used as the screening standard for significant changes in gene expression (expression up-regulation), and combined with WEGO and KEGG for gene function prediction and metabolic pathway prediction analysis, the preliminary screening obtained 3 functions that obviously respond to potato virus Y virus disease. Genes (Ntab0224260, Ntab0691000, Ntab0319080).
[0052] Based on the existing tobacco genome data, the Ntab0224260 and Ntab0691000 gene seq...
Embodiment 2
[0096] On the basis of Example 1, in order to facilitate the practical application of early-warning gene detection, further, the inventor prepared a detection kit in combination with other consumables and reagents, so as to facilitate the convenient and rapid development of related detection work. The detection kits are mainly experimental reagents, and can also be designed to include several instrument consumables, specifically:
[0097] Instrument consumables include: 10 μL, 200 μL and 1000 μL pipette tips (DNase-free, RNase-free) each, 1.5 mL centrifuge tubes (DNase-free, RNase-free), 1 mortar, and several pairs of disposable latex gloves , 1 Marker pen, several 96-well plates (without DNase and RNase) for fluorescence quantitative PCR;
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Abstract
The invention belongs to the technical field of tobacco genome analysis and tobacco cultivation, and specifically relates to the early warning gene of tobacco potato virus Y disease and application patent application matters thereof. The 1000-base sequence of the early warning gene Ntab069 is shown in SEQ ID No.2. The early warning gene provided by this application has the technical advantages of fast response and high sensitivity, and because it detects the tobacco genome (the prior art mostly detects the genome of pathogenic bacteria), it has the technical advantage of convenience. This application can not only lay a certain technical foundation for the monitoring and early prevention of potato virus Y disease, but also provide reference and reference for the prevention and control of other tobacco diseases, so it has good application and scientific research value.
Description
technical field [0001] The invention belongs to the technical field of tobacco genome analysis and tobacco cultivation, and in particular relates to a tobacco potato virus disease early warning gene and its application for patent application. Background technique [0002] In tobacco cultivation, various microorganisms such as fungi, bacteria and viruses can lead to the occurrence of tobacco diseases. At the same time, insects with piercing-sucking mouthparts such as aphids can also transmit pathogens to tobacco and cause diseases. However, due to the small size of microorganisms and the diverse transmission routes of tobacco diseases, it is difficult to study the occurrence and prevalence of tobacco diseases. In the prior art, indicators such as disease index are generally used to reflect the occurrence, development and epidemic law of diseases, but this evaluation method usually cannot judge the occurrence or prevalence of diseases until the disease occurs or even begins to...
Claims
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Application Information
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