Uranyl ion titration detection method and device based on symmetrical optical fiber taper biosensing

Abstract

The invention discloses a UO2<2+> titration detection method and device based on symmetrical optical fiber taper biosensing. The device comprises a light source, a probe, a UO2<2+> titration reactiontank, a photoelectric conversion module and a data processing module, the probe is a symmetrical optical fiber taper biological probe, i.e., an optical fiber is used as a probe carrier to modify39 DNAzyme on the surface of the symmetrical optical fiber taper through a chemical method to prepare the biosensor. The fiber cone biological probe is arranged in a titration area of the UO2<2+> titrationreaction tank to realize titration method detection; and the light source is a laser light source, an evanescent wave is used as exciting light, fluorescence intensity is used as a test object, and anoptical signal is used as a medium to realize detection of a UO2<2 +> sample. According to the invention, the concentration of UO2<2+> in water is calculated by detecting the intensity of output fluorescence, so that the problems that the existing UO2<2+> detection instrument in China is large in size and tedious to operate and is difficult to meet the field portable detection requirement can besolved.

Description

technical field [0001] The invention relates to the fields of optical fiber biosensing and environmental water pollution detection, in particular to a method based on optical fiber biosensing for detecting UO in outdoor environment water. 2 2+ detection methods and devices. Background technique [0002] During the mining and smelting of uranium ore and the operation of nuclear facilities, waste water with natural radionuclide uranium will be produced, which will seriously harm the ecological environment. At present, the waste rock and tailings of uranium tailings ponds in my country have reached tens of millions of tons, mainly distributed in densely populated areas such as Jiangxi, Hunan, and Guangdong. The diffusion of radionuclides produced by these tailings has the characteristics of long impact period and high concealment, which poses a serious threat to the safety of water bodies in the surrounding environment. Under the basic national policy of my country's green e...

AI Technical Summary

Problems solved by technology

[0008] However, none of the above-mentioned test methods has been applied to field portable detection or automatic detection so far.

Analysis of the reasons found that these methods are cumbersome, rely on manual operation in the laboratory, and need to be completed under strict experimental environment and hardware conditions, which cannot meet the portable requirements in the field, and are difficult to carry out automated applications

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