Glycosaminoglycan composition and preparation method and application thereof

A glycosaminoglycan and composition technology, applied in the field of glycosaminoglycan composition and its preparation, can solve problems such as easy risk, complicated supply chain, affecting the quality or supply of heparin products, and achieve prevention and repair of vascular lesions , significant economic effect, and low bleeding risk

Pending Publication Date: 2020-12-15
SUZHOU RONGXI BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in the current U.S. market, its heparin products (new drugs, generic drugs, devices, etc.) all come from the intestinal mucosa of pigs. However, 75% of the crude products used to produce high-quality heparin come from China. There is a serious problem of single animal and regional sources. Risk control (such as pig blue-ear disease and other epidemics, wars, trade disputes, etc., especially the recent large-scale outbreak of African swine fever in China), which seriously affects the quality or ...

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  • Glycosaminoglycan composition and preparation method and application thereof
  • Glycosaminoglycan composition and preparation method and application thereof
  • Glycosaminoglycan composition and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1: Preparation of Glycosaminoglycan Composition 1

[0046] The by-product glycosaminoglycan of sheep intestinal mucosa heparin is obtained in the process of purification and preparation of sheep intestinal mucosa heparin. Specifically include the following steps, crude sheep heparin sodium (manufacturer: Shandong Shenlian Biotechnology Co., Ltd., batch number: 20150326, obtained from sheep intestinal mucosa extraction), heparin purification adopts a well-known process in the industry, that is, dissolve the alkali in water and then dissolve the alkali hydrolyzate, and protease hydrolysis, after the hydrolyzate is adjusted to a weak base, it is adsorbed and eluted with anion exchange resin, and alcohol precipitation is used to prepare sheep heparin, and the by-product glycosaminoglycan of sheep heparin is obtained after separation of heparin. Add ethanol to the supernatant to a final mass concentration of 70%, recover the precipitate, and dry it.

[0047] Weigh 8...

Embodiment 2

[0048] Example 2: Preparation of Glycosaminoglycan Composition 2

[0049] Take the by-product glycosaminoglycan in the purification of bovine intestinal mucosal heparin, which is obtained when the crude bovine intestinal mucosal heparin is refined and purified (crude bovine intestinal mucosal heparin is extracted from the small intestine mucous membranes), obtained from the glycosaminoglycan-rich supernatant during the heparin process, obtained by precipitation with a final concentration of 70% ethanol and dried.

[0050] Weigh 100 g of bovine intestinal mucosal heparin by-product glycosaminoglycan, stir and dissolve with 1000 mL of 2% saline, then add 40 g of sodium hydroxide, and react at 25°C for 2 hours. Adjust the pH to 7.0 with dilute hydrochloric acid, add 1 g of potassium permanganate, and stir for 30 minutes. Then add 100 g of diatomaceous earth, stir for 5 min and then filter with filter paper. Add 2.2g ETDA to the filtrate, and react at room temperature for 4h. C...

Embodiment 3

[0051] Example 3: Preparation of Glycosaminoglycan Composition 3

[0052] Take 30 g of glycosaminoglycan, a by-product of bovine lung heparin, and dissolve it with 500 mL of 3% saline, then add 3 g of sodium hydroxide, and react at 35°C for 2 hours. Adjust the pH to 7.8 with dilute hydrochloric acid, add 10 mL of hydrogen peroxide, stir and let stand for decolorization for 5 hours. Add 30g of diatomaceous earth, stir for 5min and then filter with filter paper. Add 1.3g ETDA to the filtrate, and react at room temperature for 3.5h. Adjust the pH to 5.5, add cold acetone to a final concentration of 41%, stir well and then centrifuge to collect the precipitate. Dissolve the precipitate with 300 mL of 5% sodium chloride solution, adjust the pH to 5.8, add ethanol to a final concentration of 48%, and let stand overnight at room temperature. The supernatant was discarded by centrifugation, the precipitate was dehydrated with 95% ethanol, and dried in vacuum to obtain 14.2 g of the...

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Abstract

The invention provides a glycosaminoglycan composition and a preparation method and application thereof. The composition is extracted and prepared from a ruminant heparin byproduct glycosaminoglycan,consists of fast-moving heparin and dermatan sulfate, and has anticoagulation and antithrombosis effects, the antithrombin III-dependent anti-Xa activity is 60-100 U/mg, the heparin cofactor II-dependent anticoagulation activity is 100-200 U/mg, and the sheep plasma method anticoagulation activity is 50-80 U/mg. The composition can be developed into a medicine or a health care product, and has halal property. In addition, organs of ruminants such as sheep and cattle, and glycosaminoglycan as a by-product of heparin production, as raw materials of the composition are simple, convenient and easily available, and thereby economic effects are remarkable.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a glycosaminoglycan composition and a preparation method thereof. Background technique [0002] Glycosaminoglycans (GAG), a natural unbranched long-chain polysaccharide polymer composed of repeating disaccharide units containing hexuronic acid and hexosamine, are widely distributed in animals and humans. in each organ. Glycosaminoglycans are acidic mucopolysaccharides with negatively charged carboxyl or sulfate groups on the sugar chains. Glycosaminoglycans mainly include heparin (HP), heparan sulfate (HS), chondroitin sulfate (CS) and dermatan sulfate (Determa Sulfate, DS). The residues, the type of linkages between residues, and the number and position of sulfate groups vary. Glycosaminoglycans have extremely important physiological functions. In higher animals or humans, dermatan sulfate is the main component of proteoglycans in the outer layer of blood ves...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61K31/727A61P7/02A61P9/10
CPCA61K31/727A61K31/737A61P7/02A61P9/10A61K2300/00
Inventor 金永生孙军亭靳彩娟周巧云钱建根姚亦明
Owner SUZHOU RONGXI BIOTECH CO LTD
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