Lactic acid bacteria enrichment culture medium suitable for industrial production and production process thereof

A technology suitable for industrial production and enrichment culture medium, applied in the direction of microbial-based methods, using added compounds to stimulate growth, bacteria, etc., can solve the problems of high cost and increased feed production cost, and reduce production costs and reduce costs , The effect of simple operation process

Pending Publication Date: 2020-12-15
辽宁九州生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the culture of lactic acid bacteria adopts MRS broth medium, which mainly uses peptone, beef, yeast powder, glucose, etc. Broth medium contains animal-derived components, whic

Method used

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  • Lactic acid bacteria enrichment culture medium suitable for industrial production and production process thereof
  • Lactic acid bacteria enrichment culture medium suitable for industrial production and production process thereof
  • Lactic acid bacteria enrichment culture medium suitable for industrial production and production process thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A kind of production technology of the lactic acid bacteria enrichment culture medium suitable for industrial production, comprises the steps:

[0030] (1) Add water to the activation tank, control the water temperature at 35±2°C, add 400g of glucose, 430g of soybean meal hydrolyzate, 19.8g of carrot powder, 15.2g of potato powder, 15g of vitamin C, 31.6g of dipotassium hydrogen phosphate, Potassium dihydrogen phosphate 43.4g, magnesium sulfate 4.8g, manganese sulfate 0.5g are mixed and stirred, and the aqueous solution that is mixed with weight concentration is 1.8%;

[0031] (2) Insert 900g of powdered compound lactic acid bacteria agent into the aqueous solution of step (1), activate it for more than 12 hours at a temperature of 35±2°C, and use it for more than 12 hours, wherein the compound lactic acid bacteria agent includes 10g of compound lactic acid bacteria and 890g of carrier , compound lactic acid bacteria include 0.8g of Lactobacillus plantarum, 1.8g of Lact...

Embodiment 2

[0034] A kind of production technology of the lactic acid bacteria enrichment culture medium suitable for industrial production, comprises the steps:

[0035] (1) Add water to the activation tank, control the water temperature at 35±2°C, add glucose 410g, soybean meal hydrolyzate 425g, carrot powder 21.5g, potato powder 16.5g, B vitamins 13g, dipotassium hydrogen phosphate 32.4 g, potassium dihydrogen phosphate 44.6g, magnesium sulfate 4.5g, manganese sulfate 0.6g are mixed and stirred, and are mixed with the aqueous solution that weight concentration is 1.8%;

[0036] (2) Insert 900g of powdered compound lactic acid bacteria agent into the aqueous solution of step (1), activate it for more than 12 hours at a temperature of 35±2°C; wherein the compound lactic acid bacteria agent includes 10g of compound lactic acid bacteria and 890g of carrier , compound lactic acid bacteria include 0.8g of Lactobacillus plantarum, 1.8g of Lactobacillus casei, 0.8g of Streptococcus thermophilu...

Embodiment 3

[0039] A kind of production technology of the lactic acid bacteria enrichment culture medium suitable for industrial production, comprises the steps:

[0040] (1) Add water to the activation tank, control the water temperature at 35±2°C, add 419g of glucose, 419g of soybean meal hydrolyzate, 25.2g of carrot powder, 16.8g of potato powder, 11g of B vitamins, and 42g of dipotassium hydrogen phosphate , Potassium dihydrogen phosphate 42g, magnesium sulfate 4.19g, manganese sulfate 1.05g are mixed and stirred, and the aqueous solution that is mixed with weight concentration is 2.0%;

[0041] (2) Insert 1000g of powdered compound lactic acid bacteria agent into the aqueous solution of step (1), activate it for more than 12 hours at a temperature of 35±2°C; wherein the compound lactic acid bacteria agent includes 10g of compound lactic acid bacteria and 990g of carrier , the compound lactic acid bacteria include 0.8g of Lactobacillus plantarum, 1.8g of Lactobacillus casei, 0.8g of S...

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Abstract

The invention discloses a lactic acid bacteria enrichment culture medium suitable for industrial production and a production process thereof, and belongs to the technical field of microorganism culture media. According to the key technical points, the lactic acid bacteria enrichment culture medium is prepared from the following components in parts by weight of 400 to 430 parts of glucose, 400 to 430 parts of soybean meal zymolyte, 35 to 45 parts of growth factor, 5 to 15 parts of vitamins, 75 to 93 parts of buffer agent, 3.5 to 4.8 parts of magnesium sulfate and 0.5 to 1.5 parts of manganese sulfate, so that the culture cost of lactic acid bacteria in production is reduced. The use process of the lactic acid bacteria enrichment culture medium comprises the following steps of (1) adding water into a container, controlling the water temperature at 35 +/-2 DEG C, adding all the raw materials, and performing stirring to prepare an aqueous solution with the weight concentration of 1.8-2.5%;and (2) inoculating a powdery compound lactobacillus inoculant into the aqueous solution obtained in the step (1), and performing activating for 12 hours or above at the temperature of 35 +/-2 DEG Cfor use.

Description

technical field [0001] The application relates to the field of microbial culture medium, in particular to a lactic acid bacteria enrichment medium suitable for industrial production and a production process thereof. Background technique [0002] Lactic acid bacteria are widely distributed in nature and there are many kinds. Most of them are essential flora with important physiological functions for humans and animals. A variety of nutrients, adjust the pH value of the intestinal tract, and inhibit the reproduction of harmful microorganisms, so lactic acid bacteria have been widely used in the aquaculture industry. [0003] At present, most of the culture of lactic acid bacteria adopts MRS broth medium, which mainly uses peptone, beef, yeast powder, glucose, etc. Broth medium contains animal-derived components, which are generally not suitable for use in cattle feed. At the same time, the cost is too high when used in large quantities in production, resulting in a substantia...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12R1/25C12R1/245C12R1/46C12R1/23C12R1/01
CPCC12N1/38C12N1/20
Inventor 韩淑敏李建涛彭高望张蓉居伟
Owner 辽宁九州生物科技有限公司
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