Method for biomimetic culture of urothelial cells and uses thereof

A urothelial, cell technology, applied in urinary tract/kidney cells, cell culture active agents, biochemical equipment and methods, etc., can solve problems such as no treatment methods

Pending Publication Date: 2020-12-18
CHINA MEDICAL UNIVERSITY(TW)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently available therapies on cystitis, while effective, only provide localized solutions and relief, so there is no optimal treatment

Method used

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  • Method for biomimetic culture of urothelial cells and uses thereof
  • Method for biomimetic culture of urothelial cells and uses thereof
  • Method for biomimetic culture of urothelial cells and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0054] The following examples are non-limiting and merely represent various aspects and features of the invention.

[0055] Materials and methods

[0056] Isolation and culture of PUC

[0057] Pig bladders for urothelial cell isolation were obtained from a local slaughterhouse. Cut the bladder tissue into 1-2 cm sections 2 Tissue pieces were removed and treated with dispase II dissolved in Hank's balanced salt solution (HBSS; Gibco, Carlsbad, CA, USA) to strip the urothelium. The dissected urothelium was minced into small pieces and incubated in cell dissociation solution in HBSS (100 U / ml) with collagenase type VI (Worthington, Lakewood, NJ, USA) to disaggregate cells. Porcine epithelial cells were isolated and cultured in Matrigel-coated dishes with Dulbecco's modified Eagle's medium (DMEM) / Ham'sF12 medium supplemented with antibiotics (penicillin 100 U / ml, streptomycin 100 mg / ml amphotericin, 5 mg / ml amphotericin B), 10% fetal bovine serum (FBS), growth factors or inhib...

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PUM

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Abstract

Provided is a method for the biomimetic culture of urothelial cells from mammalian bladders, including murine, porcine, bovine and human sources to isolate and expand urothelial cells for use in various applications, such as intravesical urothelial cell therapy to treat cystitis and bladder cancer.

Description

technical field [0001] The present invention relates to methods and compositions for isolating and expanding mammalian urothelial cells for the treatment of bladder-related diseases. In particular, the present invention relates to the use of a biomimetic culture system to obtain urothelial cells with proliferative potential, and the use of the isolated cells in a matrixed solution for the treatment of urothelial dysfunction. More particularly, the present invention relates to methods and compositions for treating bladders with urothelial damage, such as cystitis and urothelial carcinoma, to repair, restore and repair immune system and urothelial function. In particular, the present invention relates to novel techniques and uses of heterogeneous cell sources for repairing damaged urothelium and restoring the immune system for the treatment of cystitis and urothelial cancer. Background technique [0002] Injury to the urothelium, such as cystitis and urothelial cancer, can ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61P13/10
CPCA61P13/10C12N2501/115C12N5/0685C12N2501/415C12N2501/155A61P35/00A61K9/0034A61K35/22A61K45/06
Inventor 石志荣黄志平吴羣杰
Owner CHINA MEDICAL UNIVERSITY(TW)
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