Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for biomimetic culture of urothelial cells and uses thereof

A urothelial, cell technology, applied in urinary tract/kidney cells, cell culture active agents, biochemical equipment and methods, etc., can solve problems such as no treatment methods

Pending Publication Date: 2020-12-18
CHINA MEDICAL UNIVERSITY(TW)
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently available therapies on cystitis, while effective, only provide localized solutions and relief, so there is no optimal treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for biomimetic culture of urothelial cells and uses thereof
  • Method for biomimetic culture of urothelial cells and uses thereof
  • Method for biomimetic culture of urothelial cells and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0054] The following examples are non-limiting and merely represent various aspects and features of the invention.

[0055] Materials and methods

[0056] Isolation and culture of PUC

[0057] Pig bladders for urothelial cell isolation were obtained from a local slaughterhouse. Cut the bladder tissue into 1-2 cm sections 2 Tissue pieces were removed and treated with dispase II dissolved in Hank's balanced salt solution (HBSS; Gibco, Carlsbad, CA, USA) to strip the urothelium. The dissected urothelium was minced into small pieces and incubated in cell dissociation solution in HBSS (100 U / ml) with collagenase type VI (Worthington, Lakewood, NJ, USA) to disaggregate cells. Porcine epithelial cells were isolated and cultured in Matrigel-coated dishes with Dulbecco's modified Eagle's medium (DMEM) / Ham'sF12 medium supplemented with antibiotics (penicillin 100 U / ml, streptomycin 100 mg / ml amphotericin, 5 mg / ml amphotericin B), 10% fetal bovine serum (FBS), growth factors or inhib...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided is a method for the biomimetic culture of urothelial cells from mammalian bladders, including murine, porcine, bovine and human sources to isolate and expand urothelial cells for use in various applications, such as intravesical urothelial cell therapy to treat cystitis and bladder cancer.

Description

technical field [0001] The present invention relates to methods and compositions for isolating and expanding mammalian urothelial cells for the treatment of bladder-related diseases. In particular, the present invention relates to the use of a biomimetic culture system to obtain urothelial cells with proliferative potential, and the use of the isolated cells in a matrixed solution for the treatment of urothelial dysfunction. More particularly, the present invention relates to methods and compositions for treating bladders with urothelial damage, such as cystitis and urothelial carcinoma, to repair, restore and repair immune system and urothelial function. In particular, the present invention relates to novel techniques and uses of heterogeneous cell sources for repairing damaged urothelium and restoring the immune system for the treatment of cystitis and urothelial cancer. Background technique [0002] Injury to the urothelium, such as cystitis and urothelial cancer, can ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61P13/10
CPCA61P13/10C12N2501/115C12N5/0685C12N2501/415C12N2501/155A61P35/00A61K9/0034A61K35/22A61K45/06
Inventor 石志荣黄志平吴羣杰
Owner CHINA MEDICAL UNIVERSITY(TW)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com