A preparation method of multilayer spiral urethral tissue engineering scaffold

Abstract

A method for preparing a multilayer spiral urethral tissue engineering scaffold, using thermoplastic degradable polymers as printing materials, using a fused deposition 3D printer to prepare a rectangular long strip embryo body with a hollow pattern, and spirally curling to obtain a spiral tube of the embryo body , after heat treatment and nesting, a double-layer scaffold composed of inner and outer embryoid body spiral tubes was obtained, and one end of the double-layer scaffold was sutured with polylactic acid sutures, and transfected when cultured sequentially on the inner wall of the outer embryoid body spiral tube Endothelial cell sheets of vascular endothelial growth factor and fibroblast growth factor, urothelial cell sheets, and smooth muscle cell sheets transfected with human platelet growth factor during culture, filled with extracellular matrix, and then sutured into the double-layer scaffold On the other end, the scaffold for urethral tissue engineering can be obtained after being fixed with thrombin. The stent prepared by the invention can keep the urethra unobstructed, is beneficial to the healing of the damaged urethral tissue, realizes the vascularization of the urethral stent in time, and can quickly realize blood supply reconstruction.

Description

technical field [0001] The invention belongs to the field of biomedical devices, and in particular relates to a preparation technology for a tissue-engineered urethral stent for treating urethral diseases such as urethral defects. [0002] Background of the invention [0003] The defect of the existing urethral stent preparation method is that although the stent formed by curling the polymer film is easy to manufacture and has high compliance, it is prone to sudden disintegration during the degradation process, forming larger fragments, which is easy to cause Urethral blockage hinders normal physiological activities of the urethra; in addition, relying solely on the self-expandability of the helical stent, the expansion rate is relatively slow, and there is a possibility of dislocation after stent implantation. The preparation process of the braided stent is slow, inefficient, time-consuming and lacks precise control of the stent structure; the compliance is insufficient and ...

AI Technical Summary

Problems solved by technology

[0003] The defect of the existing urethral stent preparation method is that although the stent formed by curling the polymer film is easy to manufacture and has high compliance, it is prone to sudden disintegration during the degradation process, forming larger fragments, which is easy to cause The urethra is blocked, which hinders the normal physiological activities of the urethra; in addition, relying solely on the self-expandability of the spiral stent, the expansion rate is relatively slow, and there is a possibility of dislocation after stent implantation

As a result, the printed product is basically in a glass state at room temperature, with low strength and high brittleness, which cannot meet the mechanical performance requirements of the bracket.

[0006] Simply implanting the carrying cells into the vicinity of the defect in the organism is prone to the problem of cell death due to insufficient supply of nutrients

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