Immunity-enhancing growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof
A technology for fermenting Chinese herbal medicines and feed additives, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of toxic side effects, poor palatability, slow effect, etc., and achieves reduced toxicity, good palatability, and reduced dosage. Effect
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[0038]In addition, the embodiments of the present invention also provide a method for preparing the above-mentioned growth-enhancing fermented Chinese herbal medicine feed additive for livestock and poultry. For reference, the preparation method may include the following steps: preparing a Chinese herbal medicine mixture into a Chinese herbal medicine fermentation medium, and The Chinese herbal medicine fermentation medium is inoculated with the seed liquid of two probiotics and fermented.
[0039]The preparation of two probiotic seed solutions of Bacillus subtilis and Bacillus licheniformis may include the following steps: inoculate Bacillus subtilis and Bacillus licheniformis in a seed medium, respectively, and then culture them at 28-37°C for 22-26h, Preferably incubate at 35°C for 24h.
[0040]The seed culture medium includes a nutrient broth solid medium and a nutrient broth liquid medium, and the ingredients include peptone, beef extract, sodium chloride, and the like.
[0041]After th...
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[0052]Example 1
[0053](1) Prepare peptone 10g / L, beef extract 5g / L, NaCl 5g / L into a nutrient broth liquid medium with a pH of 7.2, and mix peptone 10g / L, beef extract 5g / L, NaCl 5g / L, agar Powder 30g / L was prepared into a nutrient broth solid medium, sterilized at 121°C for 30 minutes, and cooled to room temperature 20°C for use.
[0054](2) Inoculate Bacillus subtilis and Bacillus licheniformis preserved in glycerol into the nutrient broth solid medium and activate them for 24 hours. Pick out the single colonies that grow well and inoculate them into the nutrient broth liquid medium. Cultivate for 24 hours under the condition of ℃, and mix the obtained Bacillus subtilis and Bacillus licheniformis according to a weight ratio of 4:6 to obtain a seed bacteria suspension of the probiotics for use.
[0055](3) 10 parts by weight of mugwort leaves, 8 parts by weight of water yellow peel, 12 parts by weight of jujube, 10 parts by weight of seaweed, and 10 parts by weight of elephant grass are p...
Example Embodiment
[0058]Example 2
[0059](1) Prepare peptone 10g / L, beef extract 5g / L, NaCl 5g / L into a nutrient broth liquid medium with pH 7.4, and mix peptone 10g / L, beef extract 5g / L, NaCl 5g / L, agar Powder 30g / L was prepared into a nutrient broth solid medium, sterilized at 121°C for 30 minutes, and cooled to room temperature 18°C for use.
[0060](2) Inoculate Bacillus subtilis and Bacillus licheniformis preserved in glycerol into the nutrient broth solid medium and activate them for 24 hours. Pick out the single colonies that grow well and inoculate them into the nutrient broth liquid medium. Cultivate for 24 hours under the condition of ℃, and mix the obtained Bacillus subtilis and Bacillus licheniformis according to a weight ratio of 4:6 to obtain a seed bacteria suspension of the probiotics for use.
[0061](3) 10 parts by weight of mugwort leaves, 8 parts by weight of water yellow peel, 12 parts by weight of jujube, 10 parts by weight of seaweed, and 10 parts by weight of elephant grass are pulv...
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