Immunity-enhancing growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof
A technology for fermenting Chinese herbal medicines and feed additives, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of toxic side effects, poor palatability, slow effect, etc., and achieves reduced toxicity, good palatability, and reduced dosage. Effect
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[0038]In addition, the embodiment of the present invention also provides a method for preparing the above-mentioned fermented Chinese herbal medicine feed additive for livestock and poultry, for reference, the preparation method may include the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation medium, and then The seed solution of two kinds of probiotics is inoculated in the Chinese herbal medicine fermentation medium, and fermented.
[0039] The preparation of two probiotic seed solutions of Bacillus subtilis and Bacillus licheniformis may include the following steps: respectively inoculating Bacillus subtilis and Bacillus licheniformis in the seed culture medium, and then cultivating at 28-37°C for 22-26h respectively, Preferably cultured at 35°C for 24 hours.
[0040] The seed medium includes nutrient broth solid medium and nutrient broth liquid medium, and the components include peptone, beef extract and sodium chlor...
Embodiment 1
[0053] (1) Prepare peptone 10g / L, beef extract 5g / L, NaCl 5g / L to prepare a nutrient broth liquid medium with a pH of 7.2, mix peptone 10g / L, beef extract 5g / L, NaCl 5g / L, agar Powder 30g / L was prepared into nutrient broth solid medium, sterilized at 121°C for 30min, cooled to room temperature 20°C for later use.
[0054] (2) Inoculate glycerol-preserved Bacillus subtilis and Bacillus licheniformis in nutrient broth solid medium and activate for 24 hours, pick out a single colony that grows well, and inoculate them in nutrient broth liquid medium respectively, and then inoculate at 35 Cultivate for 24 hours under the condition of ℃, mix the obtained Bacillus subtilis and Bacillus licheniformis according to the weight ratio of 4:6 to obtain the seed suspension of probiotics, and set aside.
[0055] (3) Grind 10 parts by weight of Artemisia argyi, 8 parts by weight of Phamada, 12 parts by weight of Jujube, 10 parts by weight of seaweed and 10 parts by weight of Elephant Grass, a...
Embodiment 2
[0059] (1) Prepare peptone 10g / L, beef extract 5g / L, NaCl 5g / L to prepare a nutrient broth liquid medium with a pH of 7.4, mix peptone 10g / L, beef extract 5g / L, NaCl 5g / L, agar Powder 30g / L was prepared into a nutrient broth solid medium, sterilized at 121°C for 30min, cooled to room temperature at 18°C for later use.
[0060] (2) Inoculate glycerol-preserved Bacillus subtilis and Bacillus licheniformis in nutrient broth solid medium for 24 hours of activation, pick out a single colony with good growth, inoculate them in nutrient broth liquid medium, and then inoculate at 37 Cultivate for 24 hours under the condition of ℃, mix the obtained Bacillus subtilis and Bacillus licheniformis according to the weight ratio of 4:6 to obtain the seed suspension of probiotics, and set aside.
[0061] (3) Grind 10 parts by weight of Artemisia argyi, 8 parts by weight of Phamada, 12 parts by weight of Jujube, 10 parts by weight of seaweed and 10 parts by weight of Elephant Grass, and mix t...
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