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Method for creating high-lysine corn breeding material

A high-lysine, corn breeding technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of lack of high-quality high-lysine corn breeding materials, etc. Lysine content, the effect of improving efficiency

Inactive Publication Date: 2021-01-05
HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to solve the technical problem of lack of high-quality high-lysine corn breeding materials in the prior art

Method used

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  • Method for creating high-lysine corn breeding material
  • Method for creating high-lysine corn breeding material
  • Method for creating high-lysine corn breeding material

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Embodiment 1

[0029] Example 1: A method for creating high-lysine corn breeding materials

[0030] (1) Specific process for creating high-lysine corn breeding materials

[0031] Using the high-lysine donor parent CA042 (maize germplasm preserved by the Henan Academy of Agricultural Sciences) as the target gene donor, the excellent common maize inbred line Zheng C09 (the nationally approved corn germplasm bred by the Henan Academy of Agricultural Sciences Grain Crops Research Institute) The male parent of maize variety Zhengdan 2265) is the reincarnation parent, and the BC 1 F 1 ; planted according to ear row BC 1 F 1 , select 100 individual plants with good agronomic traits and genetic background close to the corresponding recurrent parents for sampling, extract DNA, and use SSR molecular marker selection O 2 o 2 Heterozygous individual plants, continue to backcross to get BC 2 F 1 seed;

[0032] sow in rows BC 2 F 1 , continue to select 100 individual plants with ...

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Abstract

The invention discloses a method for creating a high-lysine corn breeding material, and aims to solve the technical problem of lack of a high-quality high-lysine corn breeding material in the prior art. A corn material donor CA042 is used as a donor parent of an o2 gene, a corn selfing line Zheng C09 is used as a receptor parent, selfing is carried out for multiple generations after backcrossing is carried out for multiple generations, and a near-isogenic line C09 / o2 is obtained. A material identified as the O2o2 heterozygous genotype is selected to continuously backcross with a recurrent parent, and a homozygous material with the genotype of o2o2 is selected after selfing. According to the method, the near-isogenic line C09 / o2 material of which the genotype is the same as that of a doublerecessive homozygote of the high-lysine donor parent is obtained through multiple rounds of backcross and selfing by means of combining SSR marker umc1066 auxiliary selection and field backcross, andthe average lysine content is increased by 55.6-70.4% compared with that of Zheng C09 serving as the receptor parent. The rich material basis can be provided for breeding of corn varieties with excellent grain protein quality, and breeding efficiency and target gene introduction accuracy are greatly improved.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a method for creating high-lysine corn breeding materials. Background technique [0002] Corn is the largest high-yielding food crop and also a feed crop. One quarter of the plant protein consumed by humans is supplied by corn. Therefore, the level of corn production is related to the global food problem, and the quality of nutrition determines whether it can be fully and effectively absorbed and utilized. However, due to the low content of lysine and tryptophan, the limiting amino acids that cannot be synthesized by pigs and poultry in corn protein, humans and other monogastric animals cannot absorb and utilize it well, greatly reducing its nutritional quality and protein utilization. . The content of lysine in ordinary corn is only about 0.25%, while the content of lysine usually required by livestock and poultry is 0.6% to 0.8%. At present, the insufficient pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895C12N15/11
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 岳润清铁双贵刘璐徐心志
Owner HENAN ACAD OF AGRI SCI
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