Quality control system for rapidly screening SARS-CoV-2 virus nucleic acid

A virus nucleic acid, sars-cov-2 technology, applied in the field of quality control system for rapid screening of SARS-CoV-2 virus nucleic acid, can solve the problems of low positive rate of virus nucleic acid detection, reliability doubts, etc., to ensure repeatability The effect of stability, accuracy, and high sensitivity

Inactive Publication Date: 2021-01-12
合肥金域医学检验实验室有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

However, in clinical applications, the positive rate of viral nucleic acid detection is low; there are clear epidemiological evidence and pneumonia symptoms in clinical practice, but the nucleic acid detection results are still negative; this brings the reliability of the results obtained by using PCR technology to detect viral nucleic acids sex questioning

Method used

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  • Quality control system for rapidly screening SARS-CoV-2 virus nucleic acid
  • Quality control system for rapidly screening SARS-CoV-2 virus nucleic acid
  • Quality control system for rapidly screening SARS-CoV-2 virus nucleic acid

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Embodiment 1

[0037] A quality control system for rapid screening of SARS-CoV-2 virus nucleic acid: including the collection of samples to be tested, the selection of detection reagents and detection instruments, the construction of detection methods, the interpretation of detection results and the evaluation indicators of effectiveness, statistics Processing; specifically include the following steps:

[0038] Step 1: Collection of samples to be tested:

[0039] 50 cases of throat swab samples with known SARS-CoV-2 virus nucleic acid test results were provided by the Municipal Center for Disease Control and Prevention, including 7 positive samples and 43 negative samples;

[0040] Collect 1,286 nucleic acid preparation / test failure samples out of 37,250 samples tested with reagent A from February 4, 2020 to March 2, 2020;

[0041] Collect 68 suspected samples of the 2019 novel coronavirus SARS-CoV-2 ORF 1ab gene, nucleocapsid protein N gene and double-gene signal elevation;

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Abstract

The invention discloses a quality control system for rapidly screening SARS-CoV-2 virus nucleic acid. The quality control system is characterized by comprising the collection of a sample to be detected, the selection of a detection reagent and detection instrument, the construction of a detection method, the interpretation of a detection result, evaluation indexes of detection result effectiveness, and statistical treatment. The quality control system disclosed by the invention adopts a fluorescent RT-PCR method, is strong in specificity, high in sensitivity, rapid in detection and accurate inresult, and is suitable for early rapid screening and detection of viruses. Meanwhile, the sample quality before detection is strictly controlled, so that the reliability of the detection result is improved; secondly, the strictly-standardized laboratory operation and quality control for guaranteeing operation precision is an important factor for guaranteeing that the detection result is accurateand repeatable; and finally, under the condition that any single-gene or double-gene detection signal is lifted abnormally, the repeated detection must be carried out to ensure the reliability of theresult and avoid missed detection.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection in molecular biology, and in particular relates to a quality control system for rapidly screening SARS-CoV-2 virus nucleic acid. Background technique [0002] Coronaviruses are a class of linear single-stranded positive-sense RNA viruses that widely exist in nature, including HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV and MERS-CoV, which can cause colds and Middle East Respiratory Syndrome severe acute respiratory syndrome (SARS) and severe acute respiratory syndrome (SARS). The 2019 novel coronavirus is a new type of coronavirus, which was named SARS-CoV-2 by the International Committee on Taxonomy of Viruses, and was named 2019-nCoV by the World Health Organization earlier. Coronavirus. [0003] In February 2020, the National Health and Medical Commission announced No. 1 that the new coronavirus-infected pneumonia was included in the "Law of the People's Republic of Ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12R1/93
CPCC12Q1/6851C12Q1/701C12Q2531/113C12Q2545/113C12Q2563/107
Inventor 李晓华李志强余娟平常中宝申梦来
Owner 合肥金域医学检验实验室有限公司
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