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A kind of Fujian rosette fern in vitro preservation medium and in vitro preservation method

An in vitro preservation, Fujian rosette technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as unreported, and achieve the effect of strong scientificity and simple experimental operation.

Active Publication Date: 2022-02-18
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the in vitro preservation of Fujian rosette fern tissue culture technology

Method used

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  • A kind of Fujian rosette fern in vitro preservation medium and in vitro preservation method
  • A kind of Fujian rosette fern in vitro preservation medium and in vitro preservation method
  • A kind of Fujian rosette fern in vitro preservation medium and in vitro preservation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Explant acquisition and inoculation

[0034] In September-October, the fujian rosette fern pinnae ( figure 1 In a), complete sporangia are removed under a dissecting microscope and loaded into a sterilized DNA purification column, the number of sporangia taken is 40 / DNA purification column. In the ultra-clean workbench, soak the collected explants in 75% ethanol for 20s, sterilize with 0.2% mercuric chloride for 4min, rinse with sterile water for 4-5 times, add sterile water and puncture the sporangia to make spores Suspension, inoculate into spore germination medium (1 / 10MS+20g / L sucrose+6g / L agar, pH 5.8), inoculate 10 bottles, culture temperature 25±2℃, light intensity 1000lx, light time 12h / d. After 15 days, the spores germinated ( figure 1 Middle b), after 30 days, the germination rate of the spores can reach 67.32%, and the spores will develop into young prothallus in about 50 days.

[0035] 2. Prothallus Proliferation

[0036] Transfer the prothallus to ...

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Abstract

The invention provides a Fujian rosette fern in vitro preservation medium and an in vitro preservation method, and relates to the technical field of germplasm preservation. The in vitro preservation medium of the present invention comprises the following components: Huabao No. 2 3g / L, NAA 0.5mg / L, sucrose 20g / L, activated carbon 1g / L and agar 6g / L, with a pH value of 5.8. When the in vitro preservation medium is used for in vitro preservation, the spores will germinate after 15 days of germination and culture, and the germination rate of the spores under microscope inspection after 30 days can reach 67.32%, and develop into young prothalloids in about 50 days; In the culture medium, inoculate 4 per bottle, the young prothallus continue to grow and proliferate, subculture once every 30 days, and the proliferation rate can reach 1:3; transfer the prothallus mass to the in vitro preservation medium, and inoculate each bottle 4, the prothallus are elongated, thick in the middle and thin at the edge, and the subculture period of the prothallus group can be extended to more than 1.5 years. To provide theoretical basis and technical support for the subsequent development, utilization and protection of wild germplasm resources related to Rosaceae.

Description

technical field [0001] The invention belongs to the technical field of germplasm preservation, and in particular relates to an in vitro preservation medium and an in vitro preservation method of Fujian rosette fern. Background technique [0002] Fujian rosette fern (Angiopteris fokiensis) belongs to Syncysticaceae rosette fern, a perennial herb, distributed in Zhejiang, Fujian, Jiangxi, Hubei, Guangdong, Hainan, Guangxi, Guizhou, Sichuan and Yunnan, etc., because of its rhizome The horseshoe-shaped persistent petiole (base) is arranged in a rosette shape and gets its name. Fujian Guanyin rosette has a beautiful plant shape, simple and straight, green and bright green leaves, and has a tropical charm. Its unique rosette is unique and has high ornamental value. It can be used as a foliage plant for indoor bonsai and landscaping. Its rhizomes can be eaten or extracted from starch, and have the functions of clearing away heat and detoxification, promoting dampness, relieving pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 何俊李村富李春芳
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI