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Molecular marker of powdery mildew resistance gene Pm12 of aegilops speotoides and use of molecular marker

A powdery mildew resistance gene and molecular marker technology, applied in the fields of genetic breeding science and molecular biology, can solve the problems of low stability and low resolution, and achieve high resolution, high detection accuracy and amplification stability Good results

Active Publication Date: 2021-02-02
CROP RES INST SHANDONG ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the SSR marker Xcau127 detects Pm12 and Pm21 The amplified DNA bands are heterogeneous, the resolution is low, and the stability is not high

Method used

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  • Molecular marker of powdery mildew resistance gene Pm12 of aegilops speotoides and use of molecular marker
  • Molecular marker of powdery mildew resistance gene Pm12 of aegilops speotoides and use of molecular marker
  • Molecular marker of powdery mildew resistance gene Pm12 of aegilops speotoides and use of molecular marker

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Embodiment

[0038] The wheat genetic germplasm used in the present invention Wembley Line #31 (publicly known, Miller etc. The introduction of a major gene for resistance to powdery mildew of wheat, Erysphe graminis f. sp. tritici , from Aegilops speotoides in to wheat, Triticum aestivum . In: Jorna ML, Slootmaker LAJ (eds) Cereal breeding related to integrated cereal production. Pudoc, Netherlands, pp 179-183, 1988) has the short arm of chromosome 6 (6SS) of Goatia spelta, carrying powdery mildew resistance disease gene Pm12 . Yangmai 18 (publicly known and public, bred and provided by Lixiahe Agricultural Science Research Institute, Jiangsu) is a wheat-Tumina 6VS / 6AL translocation line, carrying the powdery mildew resistance gene Pm21 . Yangmai No. 9 (publicly known and public, bred and provided by Jiangsu Lixiahe Agricultural Science Research Institute) is a variety susceptible to powdery mildew.

[0039] 1. Design and synthesis of primers

[0040] powdery mildew resistan...

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Abstract

The present invention discloses 3 molecular markers (6SS-1 to 6SS-3) for specifically tracking 6SS chromosomes of aegilops speotoides and a use method of the molecular markers, and relates to the technical field of molecular biology and genetic breeding science. Sequences of primers of the 6SS-1 marker are shown as SEQ ID NO.1 and SEQ ID NO.2, sequences of primers of the 6SS-2 marker are shown asSEQ ID NO.3 and SEQ ID NO.4, and sequences of primers of the 6SS-3 marker are shown as SEQ ID NO.5 and SEQ ID NO.6. The molecular markers can track the Pm12 gene from the aegilops speotoides, also cantrack a Pm21 gene from haynaldia villosa, can simultaneously distinguish and track the Pm12 gene and the Pm21 gene in a pyramiding breeding material, and have important practical value in assistant selection breeding and pyramiding breeding of the two genes.

Description

technical field [0001] The invention relates to the fields of molecular biology and genetic breeding science and technology, in particular to three specific tracking genes carrying resistance to powdery mildew Pm12 The 6SS chromosome-specific molecular marker of Ipespeltella speltina and its usage. Background technique [0002] Wheat powdery mildew is caused by the specialized powdery mildew ( Blumeria graminis f. sp. tritici ) caused by fungal diseases, is one of the important diseases of wheat production in my country. In recent years, the damage range of wheat powdery mildew has been continuously expanding, showing a trend of gradually strengthening from south to north. According to statistics, the average incidence area of ​​wheat powdery mildew in my country is more than 90 million mu per year. Therefore, wheat powdery mildew has become an important disease that seriously threatens my country's food production and safety. [0003] Discovering disease-resistant g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘成计健马朋涛李根英刘任糠朱姗颖何华纲
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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