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Bacillus licheniformis for producing bacitracin by introducing edd gene from external source and application thereof

A technology of Bacillus licheniformis and Bacillus licheniformis, applied in the direction of using vectors to introduce foreign genetic material, methods based on microorganisms, bacteria, etc., to achieve the effect of increasing the production of bacitracin and increasing the production of bacitracin

Active Publication Date: 2021-02-12
LIFECOME BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, there is currently no edd There are reports on the relationship between genes and bacitracin production. At the same time, there are many genes closely related to the synthesis of metabolites in Bacillus licheniformis. It is still unknown which gene is related to the production of bacitracin. The way to obtain high-yielding bacitracin engineered bacteria also needs further research

Method used

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  • Bacillus licheniformis for producing bacitracin by introducing edd gene from external source and application thereof
  • Bacillus licheniformis for producing bacitracin by introducing edd gene from external source and application thereof
  • Bacillus licheniformis for producing bacitracin by introducing edd gene from external source and application thereof

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Embodiment approach

[0026] The exogenously introduced edd gene Bacillus licheniformis for producing bacitracin, said exogenously introduced edd gene Bacillus licheniformis is: adopt the method of existing exogenous gene introduction host cell to introduce exogenous edd The recombinant bacterial strain obtained by introducing the gene into the original Bacillus licheniformis through the overexpression plasmid; the original Bacillus licheniformis is a Bacillus licheniformis producing bacitracin.

[0027] The specific construction method of exogenously introduced edd gene bacillus licheniformis of the present invention is:

[0028] 1. The specific operation steps of step (1) are:

[0029] According to E. coli str. K-12 substr. MG1655 ( Escherichia coli str. K-12 substr.MG1655) in the genomic DNA sequence edd gene sequence, design edd The upstream primer of the gene ( edd -F) and the downstream primer ( edd -R); and start with Escherichia coli The genomic DNA of str. K-12 substr. MG1655 was ...

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Abstract

The invention provides bacillus licheniformis with an externally introduced edd gene for producing bacitracin and application. The bacillus licheniformis with the externally introduced edd gene is a recombinant strain obtained by introducing the externally introduced edd gene into bacillus licheniformis through overexpression plasmids by adopting an existing method for introducing the externally introduced edd gene into a host cell. When the recombinant strain is used for fermentation production of bacitracin, the purpose of increasing the fermentation yield of bacitracin can be achieved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering bacteria transformation, in particular to an exogenously introduced edd gene Bacillus licheniformis for producing bacitracin and its application. Background technique [0002] Bacitracin (English name: Bacitracin), also known as subtilisin, subtilisin, trixitracin. It is an unstable polypeptide composed of a variety of amino acids, including A, B 1 , B 2 , B 3 、D 1 、D 2 、D 3 and E multiple components, their chemical structural formulas are as follows: [0003] [0004] Among them, component A has the highest activity and the most abundant content, and its molecular formula is C 66 h 103 N 17 o 16 S. It is a dodecapeptide containing a seven-membered ring. It contains a thiazoline ring formed by L-isoleucine and L-cysteine, an ε-NH in lysine 2 A cyclic heptapeptide structure formed by linkage between the side chain and the C-terminus of asparagine, and four D-amino acids ...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/75C12R1/10
CPCC12N9/88C12Y402/01012C12N15/75
Inventor 陈守文何鹏辉罗高样胡施颖向正威张钲蔡冬波李俊辉邱湘琪
Owner LIFECOME BIOCHEM