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A method for producing pqq by biological method

A biological method and seed technology, applied in the field of biological production of PQQ, can solve the problems of low yield, low PQQ fermentation yield, difficult to meet the biological fermentation field, etc., and achieve the effect of increasing yield

Active Publication Date: 2022-05-17
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This document mainly uses carbon source supplementation and medium addition of Fe 2+ To increase the yield of PQQ, but the fermentation yield of PQQ in this literature is still very low, it is difficult to meet the growing demand in the field of biological fermentation
[0005] In view of the problems of low yield, low efficiency, and dependence on special strains in the production of PQQ by hyphomycetes in the prior art, it is urgent to find a method for the production of PQQ by biological methods, so that the method can be quickly and efficiently produced by common hyphomycetes. Obtaining high yields of PQQ

Method used

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  • A method for producing pqq by biological method
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Embodiment 1

[0027] A biological method of manufacturing PQQ, comprising the following steps:

[0028] After activating the filament microbacterium, inoculate into the seed culture medium, after obtaining the seed solution, the seed liquid is inoculated into the fermentation medium, and the fermentation culture is obtained PQQ; the fermentation supplement is added in the fermentation culture process;

[0029] Among them, the seed medium includes: (NH 4 ) 2 SO 4 3g / L、Na 2 HPO 4 3g / L、KH2 PO 4 2g / L、MgSO 4 ·7H 2 O0.5g / L、CaCl 2 ·2H 2 O0.03g / L、MnCl 2 ·5H 2 O 0.006g / L、ZnSO 4 ·7H 2 O 0.006g / L、CuSO 4 ·5H 2 O0.0007g / L, anhydrous methanol 10mL / L and residual water. The incubation conditions of seed medium are: 25 °C, oscillating culture for 24h at 150 rpm, pH= 7-7.5.

[0030] Fermentation medium includes: (NH 4 ) 2 SO 4 2g / L、Na 2 HPO 4 2g / L、KH 2 PO 4 1g / L、MgSO 4 ·7H 2 O 0.1g / L、CaCl 2 ·2H 2 O 0.02g / L、MnCl 2 ·5H 2 O 0.002g / L、ZnSO 4 ·7H 2 O 0.002g / L、CuSO 4 ·5H 2 O0.0002g / L, KI 0.01g / L, NaCl 0.5g / L, shiitake...

Embodiment 2

[0033] A biological method of manufacturing PQQ, comprising the following steps:

[0034] After activating the filament microbacterium, inoculate into the seed culture medium, after obtaining the seed solution, the seed liquid is inoculated into the fermentation medium, and the fermentation culture is obtained PQQ; the fermentation supplement is added in the fermentation culture process;

[0035] Among them, the seed medium includes: (NH 4 ) 2 SO 4 3g / L、Na 2 HPO 4 3g / L、KH 2 PO 4 2g / L、MgSO 4 ·7H 2 O0.5g / L、CaCl 2 ·2H 2 O0.03g / L、MnCl 2 ·5H 2 O 0.006g / L、ZnSO 4 ·7H 2 O 0.006g / L、CuSO 4 ·5H 2 O0.0007g / L, anhydrous methanol 10mL / L and residual water. The incubation conditions of seed medium are: 37 °C, 150 rpm under the condition of oscillation culture for 24 h, pH = 7-7.5.

[0036] Fermentation medium includes: (NH 4 ) 2 SO 4 3g / L、Na 2 HPO 4 3g / L、KH 2 PO 42g / L、MgSO 4 ·7H 2 O 0.3g / L、CaCl 2 ·2H 2 O 0.04g / L、MnCl 2 ·5H 2 O 0.006g / L、ZnSO 4 ·7H 2 O 0.006g / L、CuSO 4 ·5H 2 O0.0006g / L, KI 0.02g / L...

Embodiment 3

[0039] A biological method of manufacturing PQQ, comprising the following steps:

[0040] After activating the filament microbacterium, inoculate into the seed culture medium, after obtaining the seed solution, the seed liquid is inoculated into the fermentation medium, and the fermentation culture is obtained PQQ; the fermentation supplement is added in the fermentation culture process;

[0041] Among them, the seed medium includes: (NH 4 ) 2 SO 4 3g / L、Na 2 HPO 4 3g / L、KH 2 PO 4 2g / L、MgSO 4 ·7H 2 O0.5g / L、CaCl 2 ·2H2 O0.03g / L、MnCl 2 ·5H 2 O 0.006g / L、ZnSO 4 ·7H 2 O 0.006g / L、CuSO 4 ·5H 2 O0.0007g / L, anhydrous methanol 10mL / L and residual water. The incubation conditions of seed medium are: 30 °C, 150 rpm under the condition of oscillating culture for 24 h, pH = 7-7.5.

[0042] Fermentation medium includes: (NH 4 ) 2 SO 4 2.5g / L、Na 2 HPO 4 2.5g / L、KH 2 PO 4 1.5g / L、MgSO 4 ·7H 2 O0.2g / L、CaCl 2 ·2H 2 O 0.03g / L、MnCl 2 ·5H 2 O 0.005g / L、ZnSO 4 ·7H 2 O 0.005g / L、CuSO 4 ·5H 2 O0.0005g / L, KI 0.0...

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Abstract

The invention provides a method for producing PQQ by a biological method, which relates to the technical field of biological fermentation. The method comprises the following steps: inoculating the hyphaetic microbes into the seed culture medium after activating them, and inoculating the seed liquid into the fermentation medium after obtaining the seed liquid , fermentation culture obtains PQQ; Add fermentation additive in fermentation culture process; Described fermentation medium comprises: (NH 4 ) 2 SO 4 、Na 2 HPO 4 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 O, MnCl 2 ·5H 2 O, ZnSO 4 ·7H 2 O, CuSO 4 ·5H 2 O, KI, NaCl, lentinan, wolfberry polysaccharide, defoamer, anhydrous methanol and the rest of water. By further adding components such as methanol, glycerin, wolfberry polysaccharide, lentinan and water-soluble soybean isoflavones during the fermentation process, it is helpful to promote the fermentation process and increase the PQQ yield.

Description

Technical field [0001] The present invention relates to the field of biological fermentation technology, specifically to a biological method of manufacturing PQQ. Background [0002] Pyrroloquinolinequinone (PQQ) is a water-soluble quinone compound, which is a coenzyme of class 3 oxidoreductase found after nicotinamide nucleotides and flavin nucleotides, which can prevent living cells from being damaged by oxygen in vivo or in vitro, and can also be used as a nutritional factor and vitamin to promote cell growth and improve the tolerance of bacteria under extreme conditions, which is of great significance. Due to the complexity of PQQ's chemical synthesis method and many by-products, more and more researchers are paying attention to its biomanufacturing methods and carrying out a series of studies. So far, the research has found a variety of PQQ-producing strains, including filamentous microbacteria, methyl nutrient bacteria, yellow bacillus, glucobacterium oxide, Acinetobacter ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18C12N1/20C12R1/01
CPCC12P17/182C12N1/20
Inventor 钟迎东张海军马贵军王国强
Owner 内蒙古拜克生物有限公司