Method for judging proper amplitude and optimal concentration of plant growth bicarbonate
A bicarbonate, plant growth technology, applied in the fields of botanical equipment and methods, horticultural methods, testing plants/trees, etc. The effect of environmental optimization and biodiversity increase
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Embodiment 1
[0031] In this experiment, mulberry, Zhugecai and rapeseed were selected, and the seedlings were raised in the soil of the greenhouse. After two true leaves grew, the plants were cultivated with the normal formula of Hoagland nutrient solution, and they were randomly divided into groups for measurement after 15 days.
[0032] Respectively select the second expanded leaves of the plants that were cultured in Hoagland nutrient solution that added different concentrations of bicarbonate for 10 days, 20 days, 30 days, 40 days, and 50 days, and use the Li-6400XT portable photosynthetic measurement system instrument (LI-6400Lincoln , NE, USA) to measure the net photosynthetic rate. Add different bicarbonate amounts in Hoagland nutrient solution to set the bicarbonate treatment of different concentrations, bicarbonate is with sodium bicarbonate (NaHCO 3 ) was added in the form of 4 treatments in total: control (CK, 0mM, no HCO 3 - ), 1 mM, 5 mM and 10 mM. Use 1mol L for the pH of ...
Embodiment 2
[0043] In this experiment, the seeds with full mulberry seeds were selected and placed in a seedling box filled with a certain volume of perlite. The seeds were covered with a thin layer of perlite. A certain amount of distilled water was injected into the water box of the seedling tray to prevent the seeds It is advisable to keep the temperature of the culture room at 25°C and light for 12 hours. About 12 days later, the seeds began to germinate. When the seedlings had 4 leaves, select the vigorous seedlings and transplant them into 12-hole seedling boxes. Cultivate 2 seedlings in each seedling box and keep an appropriate distance to ensure that the seedlings grow to a suitable experimental size. Hours do not obscure each other in light. After transplanting the seedlings, place the seedling box with the plants in the artificial climate chamber, set the photoperiod to 12 h, and the light intensity to 300 μmol m -2 the s -2 For PPFD, the daytime temperature is maintained at...
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