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Method for auxiliary screening of tomato materials with high-sugar character, kit and application

A kit and tomato technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of low sugar content, high sugar content, small fruit weight, etc.

Active Publication Date: 2021-03-05
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Cherry tomato is a transition type from wild tomato to cultivated tomato. It has high sugar content and small fruit weight. The fruit weight of cultivated tomato is much larger than that of cherry tomato, but its sugar content is low.

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  • Method for auxiliary screening of tomato materials with high-sugar character, kit and application
  • Method for auxiliary screening of tomato materials with high-sugar character, kit and application
  • Method for auxiliary screening of tomato materials with high-sugar character, kit and application

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Embodiment Construction

[0026] The present invention will be further described below in conjunction with the examples, but not as a limitation of the present invention. The experimental methods without specific conditions indicated in the following examples generally follow the known methods in the art.

[0027] Using traditional breeding methods to select high-sugar tomato materials is not only difficult and costly, but also easily affected by environmental conditions. In order to solve this technical problem, the present invention has developed two SNP marker identification primers associated with tomato sugar content, which can Tomato materials with high sugar traits are identified at the seedling stage, thereby speeding up the breeding process and improving breeding efficiency.

[0028] The 2 SNP sites associated with the tomato sugar content trait of the present invention are specifically obtained through the following steps:

[0029] (1) Obtaining the SNP mutation site on chromosome 6 of the t...

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Abstract

The invention discloses a method for auxiliary screening of tomato materials with high sugar character, a kit and application. Two SNP sites related to the high-sugar character of the tomato materialsare a basic group C or T at the 37559435bp position of a sixth chromosome of a tomato genome and a basic group A or G at the 3478110bp position of a ninth chromosome of the tomato genome. Through marking of the two SNP sites, the method for auxiliary screening of the tomato material with the high-sugar character as well as the application of the SNP sites at the 37559435bp position of the sixth chromosome of the tomato genome and at the 3478110bp position of the ninth chromosome of the tomato genome to molecular marker-assisted selective breeding of tomatoes are disclosed. High-sugar tomato materials can be identified in the seedling stage, so that the breeding process is accelerated, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a method, kit and application for assisting in screening tomato materials with high-sugar traits. Background technique [0002] The sugar content in tomato fruit is one of the important goals in tomato quality breeding, because the sugar content in tomato fruit has an important impact on fruit flavor, color and nutrient content, and is the main factor determining the quality of tomato fruit. The sugar content of tomato fruit is also very important for processed tomatoes. The quality of processed tomato pulp, jam and juice depends largely on the type and content of sugars accumulated in the fruit. The sugar content in tomato fruit is a quantitative trait. Many researchers have conducted a large number of QTL (quantitative trait loci) analyzes on this trait, and found that most of the QTL loci can explain a relatively low phenotypic variation rate, and most...

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨学东朱为民朱龙英张雪莲冯岩张辉张迎迎刘雅慧
Owner SHANGHAI ACAD OF AGRI SCI