DCAPS (Derived Cleaved Amplified Polymorphic Sequence) marker development for identifying flooding tolerance of chrysanthemum and application
A waterlogging tolerance, chrysanthemum technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of complex genetic background, restricted genome research and development, difficult chrysanthemum breeding work, etc., to increase accuracy performance, improve accuracy
Active Publication Date: 2018-03-20
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
[0004] Chrysanthemum is a highly heterozygous allopolyploid, and the complex genetic background limits the development of its genome research
Most of the molecular markers currently used in chrysanthemums are dominant markers, and it is difficult to directly apply them in chrysanthemum breeding
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[0056] The present invention will be further described below in conjunction with the examples, and the experimental methods that do not specify specific conditions in the following examples generally follow the known methods in the art.
[0057] (1) Mining SNPs significantly associated with waterlogging tolerance of chrysanthemum
[0058](1) Acquisition of test materials and waterlogging phenotype data: 88 chrysanthemum resources from different sources and not directly related were selected as test materials, and all materials were preserved in the "China Chrysanthemum Germplasm Resources Preservation Center". The above-mentioned germplasm was obtained from the "China Chrysanthemum Germplasm Resource Conservation Center". The potted simulated flooding method was used to conduct three waterlogging tolerance identification tests on the natural population at the seedling stage of chrysanthemums. The flooding treatment was kept 3cm above the water surface. The wilting index was co...
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The invention relates to a dCAPS (Derived Cleaved Amplified Polymorphic Sequence) marker development method for identifying flooding tolerance of chrysanthemum and application, which belong to the technical field of biology. The method comprises the following steps of a, digging SNP (Single Nucleotide Polymorphism) loci remarkably related to the flooding tolerance of the chrysanthemum through a GWAS (Genome Wide Association Study) method; b, analyzing SNP mutation loci specific enzyme cutting loci, and designing a dCAPS primer; c, predicating cleaved amplified polymorphism by combining genotype and phenotype data; d, verifying a dCAPS marker in a natural flooding tolerance extreme crowd; e, further verifying a WT-dCAPS1 marker in an F1 offspring flooding tolerance extreme crowd. The invention develops a dCAPS co-dominance marker co-separated from a flooding tolerance character of the chrysanthemum, the marker is named as the WT-dCAPS1, and the average verification accuracy is 78.9 percent in two crowds, so that the marker is preliminarily considered to be applied to chrysanthemum flooding tolerance molecular marker assistant breeding, beneficial to greatly shortening a breeding period, and has important theoretical and practical significance on cultivating new species of chrysanthemum with flooding tolerance.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a dCAPS marker development method and application for identifying the waterlogging tolerance of chrysanthemums, which can be used for assisted breeding of chrysanthemums waterlogging tolerance molecular markers. Background technique [0002] Waterlogging is a serious natural disaster that endangers the production of chrysanthemums in the world. Breeding new varieties of chrysanthemums with waterlogging tolerance is a direct and effective way to solve this problem. Therefore, it is of great significance to develop specific molecular markers related to waterlogging tolerance of chrysanthemum. At present, traditional molecular markers such as RAPD, AFLP, SRAP, ISSR, and SSR have been successfully applied in the identification of chrysanthemum germplasm resources, genetic diversity analysis, linkage map construction and association analysis [1-5] . With the development of modern molecula...
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Login to View More IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 陈发棣苏江硕张飞种昕冉宋爱萍房伟民陈素梅蒋甲福
Owner NANJING AGRICULTURAL UNIVERSITY