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dcaps molecular markers for identification of rice ear length traits and their applications

A molecular marker, rice technology, applied in the field of molecular biology, can solve the problem of low probability of discrimination and recognition

Active Publication Date: 2020-09-18
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low probability of naturally occurring recognition by restriction endonucleases, the development of CAPS is limited

Method used

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  • dcaps molecular markers for identification of rice ear length traits and their applications
  • dcaps molecular markers for identification of rice ear length traits and their applications
  • dcaps molecular markers for identification of rice ear length traits and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 is used to identify the development of dCAPS molecular markers of rice panicle length traits

[0038] 1. Mining SNP loci significantly associated with rice long panicle traits by SLAF-Seq method

[0039] (1) Select two rice cultivars from different sources and have significant differences in panicle length traits to construct a population of recombinant inbred lines (RILs) by crossing G1025 (short panicle) and K1561 (long panicle);

[0040] (2) Using the specific site amplified fragment (SLAF) sequencing technology, a high-density genetic linkage map was constructed using the genotype data of the RILs population;

[0041] (3) Based on the genetic linkage map, the quantitative trait loci (QTL) of panicle length in the RILs population were analyzed, and a main effect QTL (called PL9) controlling panicle length was detected, and obtained the long panicle of rice. SNP loci significantly associated with traits;

[0042] 2. SNP site-specific restriction enzyme sit...

Embodiment 2

[0052] Example 2 Verification of dCAPS molecular markers for identifying rice panicle length traits

[0053] (1) Genomic DNA of 12 short-eared rice varieties and 12 long-eared rice varieties was extracted by CTAB method. After detecting the concentration and purity of DNA with a nucleic acid analyzer, the ddH 2 O is diluted to 300ng / L for subsequent use;

[0054] (2) Use the specific primer pairs designed in Example 1 to perform PCR amplification on 24 materials. The 20 μL PCR amplification reaction system is: 1 μL of genomic DNA to be tested, 0.5 μL of upstream and downstream primers, 10 μL of 2×Es Taq DNA Polymerase Reactionmix (TIANGEN, Beijing), 8 μL of distilled water. The PCR amplification reaction program is: denaturation at 94°C for 5 minutes; 30 cycles at 94°C for 30s, 57°C for 30s, and 72°C for 30s; extension at 72°C for 5 minutes;

[0055] (3) After the PCR reaction, 10 μL of the reaction product was detected by 2% agarose gel electrophoresis, and a single band wa...

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Abstract

The invention provides a dCAPS molecular marker for identifying the ear length character of rice and application thereof. The dCAPS molecular marker is a PL-dCAPS marker. 316bp bands can be amplifiedby using primers in rice genome DNA, and only one band exists after rice amplification bands of a long-ear variety are subjected to enzyme digestion with restriction enzyme Xba I; and two bands are obtained after rice amplification bands of a short-ear variety is subjected to enzyme digestion with the restriction enzyme Xba I, and a specific primer sequence for amplifying the molecular marker is shown as SEQ.ID.NO.1-2. The molecular marker for identifying the ear length character of rice provided by the invention can be applied to molecular marker-assisted rice breeding with a high-yield character, can quickly screen out rice with the long-ear character without field statistics, avoids the influence of environmental factors on phenotypic identification, greatly shortens the breeding period, and has important theoretical and practical significance for cultivating new rice varieties with a long-ear character.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a dCAPS molecular marker for identifying rice panicle length traits and its application. Background technique [0002] Rice (Oryza sativa L.) is an important food crop that feeds more than half of the world's population. my country is a big producer and consumer of rice, so rice plays a vital role in food production and security in my country and the world. The growth and development of rice and the physiological regulation process affect the final yield and quality. Therefore, the use of molecular biology techniques to breed rice to enhance quality and increase yield has become an effective way to improve rice production. [0003] The yield of rice is closely related to various rice panicle traits. Spike length is a selection index for panicle traits that is positively correlated with rice yield. Cloning the gene that controls rice panicle length can help to understand ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 郭嗣斌李孝琼韦宇陈颖刘开强
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI