A dcaps molecular marker for rapid identification or auxiliary identification of tomato soluble solids and its application
An auxiliary identification and soluble technology, which is applied in the fields of genetic engineering and molecular biology, can solve the problems of narrow genetic background of tomato cultivated populations, and achieve the effect of increasing practicality and accuracy
Active Publication Date: 2022-06-21
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology
Tomato is a self-pollinated crop. After long-term selection and domestication, the genetic background of tomato cultivated populations has become narrower and narrower. It is difficult to achieve great results in the study of soluble solids traits using existing genetic breeding resources and conventional breeding methods. progress
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[0034] Below in conjunction with embodiment, the present invention will be further described, the experimental method of unreceipted specific conditions in the following embodiment, usually according to well-known means in the art.
[0035](1) Acquisition of SNP mutation sites
[0036] Reference to known literature: Petit, J. (2014). Master thesis: Identification of introgression region(s) related to brix content with increased fruit soluble solids. Wageningen university. The upstream primer sequence was designed according to the standard SNPJCF: 5'-CCGACTTGTCATCAATACTGTG-3'( SEQ ID NO: 1) and the downstream primer sequence SNPJCR: 5'-GTTCTATTTGATGCTGTATGGG-3' (SEQ ID NO: 2), for 204 breeding materials with different known soluble solid contents (the test materials were from Shanghai Academy of Agricultural Sciences, soluble The solid content was obtained by taking the overall average value of the strains of the variety) for PCR amplification and then sanger sequencing. Throug...
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The invention discloses a SNP marker related to tomato soluble solids. The SNP marker is located at the base C or T at the 37559435 bp of chromosome 6 of the tomato genome. The invention also discloses a dCAPS molecular marker assisted breeding method for rapid identification or auxiliary identification of tomato soluble solids. The SNP site is used as a dCAPS marker for identifying tomato soluble solids content, and the marker is named BR1-dCAPS marker . The present invention also provides a method for rapidly identifying or assisting identification of tomato soluble solid content, through the detection of the SNP marker on the tested tomato, identifying or assisting identification of tomato soluble solid content. The present invention is applied to tomato soluble solid content related breeding and assisted breeding, and can quickly screen and obtain tomato soluble solid material with different content, which not only avoids the influence of environmental factors on phenotype identification, but also greatly shortens the breeding cycle. Fast and effective advanced breeding methods.
Description
technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a dCAPS marker and application for rapid identification or auxiliary identification of tomato soluble solids. Background technique [0002] Tomato soluble solids are mainly composed of nutrients such as soluble sugars and organic acids. Tomato soluble solids are an important indicator to measure tomato fruit quality and yield. The level of soluble solids directly affects the flavor quality of tomatoes. For processed tomatoes, every 1% increase in soluble solids is equivalent to a 25% increase in total yield. At the same time, soluble solids play an important role in plant stress resistance. Tomato is a self-pollinating crop. After long-term selection and domestication, the genetic background of the tomato cultivated population has become narrower and narrower. It is difficult to achieve great results in the study of soluble solids using existing genetic ...
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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨学东朱为民张雪莲苏晓梅冯岩张辉张迎迎朱龙英
Owner SHANGHAI ACAD OF AGRI SCI