Mutant of xylanase xyna and its application

Abstract

The invention provides a mutant of xylanase XynA and application thereof. Compared with XynA, the optimum temperature of mutants XynAS and XynAR increased by 10°C and 15°C, reaching 70°C and 75°C, respectively, and the residual enzyme activity of XynAR still reached more than 90% when incubated at 80°C for 30 minutes, and the hydrolysis of XynAS and XynAR The efficiency of xylan was improved, mainly reflected in the shortened hydrolysis time of XynAR, the significant increase of xylobiose and xylotriose yield, the shortened hydrolysis time of XynAS, and the significant increase of xylan tetraose yield; XynAN hydrolyzed beech wood and oat xylan, and the hydrolyzate The ratio is changed, and the efficiency of hydrolyzing birch xylan is improved. Therefore, the mutant of the present invention has good application value and prospect in the production of xylooligosaccharides from agricultural waste.

Description

technical field [0001] The invention belongs to the field of enzyme engineering, in particular to the mutation of xylanase and its application in producing xylooligosaccharides from agricultural waste. Background technique [0002] Agricultural waste is a general term for waste discharged from agricultural production, agricultural product processing, livestock and poultry breeding and rural residents' life. my country is a large agricultural country. With the production and processing of agricultural products, a large amount of agricultural waste is produced every year. These agricultural wastes are good renewable resources, but they have not been effectively developed and utilized, resulting in the development of my country's agriculture. There are prominent problems such as large amount of agricultural waste discharge and serious environmental pollution. The concept of "leading rural revitalization with green development" requires the full development and utilization of ag...

AI Technical Summary

Problems solved by technology

[0004] At present, xylanase is mainly used in industrial production such as papermaking, food and feed. In order to better apply to complex and diverse production environments, the characteristics of xylanase have been extensively studied, such as heat resistance, acid / alkali resistance , substrate specificity, etc., but there are few studies on enzyme-catalyzed substrates to generate specific hydrolysis products-xylooligosaccharides (mainly xylobiose and xylotriose), and the specific mechanism of hydrolysis is unclear, while GH11 xylo Carbohydrase uses low-value-added agricultural waste to produce high-value-added xylo-oligosaccharides, the key lies in the study of its specific hydrolysis mechanism

Studies have shown that the N-terminus has a certain influence on the hydrolysis specificity of GH11 xylanase

Images