A low-temperature exo-inulinase mutant mutp126r stable at mesophilic temperatures

An exo-inulinase and mutant technology, applied in the direction of enzymes, hydrolases, glycosylases, etc., can solve the problems of poor heat resistance, low-temperature enzymes are prone to heat denaturation, etc., and achieve good thermal stability
CN112725309BActive Publication Date: 2022-06-24YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2022-06-24

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Abstract

The invention discloses a low-temperature exo-inulinase mutant MutP126R stable at medium temperature. The mutant MutP126R has the amino acid sequence shown in SEQ ID NO.1, and its thermal activity and thermal stability have changed. Elevated temperature and better thermal stability are beneficial to the production, storage and transportation of enzymes. The optimal temperature of the purified wild enzyme InuAMN8 was 35°C, and that of the mutant enzyme MutP126R was 40°C; after treatment at 55°C for 10–60 min, the activity of the wild enzyme InuAMN8 decreased from 70% to 17%, and that of the mutant enzyme MutP126R Enzyme activity decreased from 70% to 26%. The low-temperature exo-inulinase mutant MutP126R of the present invention can be applied to industries such as food, brewing, and bioenergy.
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Description

technical field

[0001] The present invention relates to a low-temperature exo-inulinase mutant, in particular to a low-temperature exo-inulinase mutant MutP126R that is stable at medium temperature. Background technique

[0002] Jerusalem artichoke has very strong disease resistance, strong regeneration, easy proliferation, high yield, cold resistance, barren resistance and drought resistance. Inulin is the main component that makes up Jerusalem artichoke tubers and can account for 19% of its wet weight or 70% of its dry weight.

[0003] Inulin is a fructan with a glucose residue at the end. Exoinulinase can degrade inulin to fructose and a small amount of glucose to produce fructose syrup with a content of up to 95%. Fructose can promote the absorption of iron in children, promote the absorption of calcium in postmenopausal women, prevent obesity, and promote the formation of beneficial intestinal flora to prevent colon cancer. Fructose can also be used to produce bioetha...

Claims

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