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A primer-probe composition and kit for synchronous detection of 8 fungi and parasite-related pathogens associated with digestive tract infections in children

A technology for simultaneous detection of gastrointestinal infections, applied in the field of detection kits, to achieve the effects of rapid detection, strong primer specificity, and wide application

Active Publication Date: 2021-11-09
爱科睿特生物医疗科技(南京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no product that can perform simultaneous PCR detection of the above-mentioned fungi and parasite-related pathogens in the digestive tract of children, and an effective method to ensure the accuracy and specificity of the detection

Method used

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  • A primer-probe composition and kit for synchronous detection of 8 fungi and parasite-related pathogens associated with digestive tract infections in children
  • A primer-probe composition and kit for synchronous detection of 8 fungi and parasite-related pathogens associated with digestive tract infections in children
  • A primer-probe composition and kit for synchronous detection of 8 fungi and parasite-related pathogens associated with digestive tract infections in children

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Embodiment Construction

[0029] The kit consists of primer-probe mixture, RT-PCR buffer, mixed enzyme solution, positive quality control and RNase-free water; RT-PCR buffer includes PCR buffer, dATP, dUTP, dCTP, dGTP and MgCl 2 ; The mixed enzyme solution includes HotStart Taq enzyme, reverse transcriptase and UNG enzyme; the positive quality control contains plasmids or RAN particles corresponding to the amplified gene sequences of gastrointestinal pathogens.

[0030] The final concentration of the primer in the amplification system is 100-1000nM; the final concentration of the probe in the amplification system is 50-500nM.

[0031] The reaction system of the kit is 30 μL, specifically: 7 μL of nucleic acid template, 20 μL of RT-PCR buffer, 1 μL of mixed enzyme solution, and 2 μL of primer-probe mixture.

[0032] Kit operation and result determination:

[0033] (1) Add 7 μL each of the sample DNA / RNA co-extraction template (extracted from the stool of the patient), RNase-free water, and positive qua...

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Abstract

The invention relates to a primer probe composition and a kit for synchronous detection of 8 kinds of children's digestive tract infection fungi and parasite-related pathogens. Common fungi and parasites in systemic infections are currently the most comprehensive detection method for fungal and parasitic infections in children's digestive tract. Through the disclosure of the technical solution, the technical problem existing in the prior art that the research on the diagnosis of fungal and parasitic pathogens related to children's digestive tract infection is still insufficient can be alleviated.

Description

technical field [0001] The invention belongs to the field of molecular biology of pathogenic microorganisms, and in particular relates to a combination of primers and probes, a kit and a detection method for the serial detection and differentiation of fungal and parasitic pathogens of children's digestive tract infection. Background technique [0002] Gastrointestinal infection is a common disease, which is especially common in clinical pediatrics. The incidence rate is increasing year by year. It is second only to respiratory system infection and ranks the second most common disease in pediatrics. It is mostly caused by bacteria, viruses, fungi or parasites, etc. Caused by pathogenic infection. It can occur throughout the year, and the common fungi are Mucor (Muc), Candida albicans (MoA), Cryptosporidium (Cryp), Cyclosporidium (Cyc), Entamoeba histolytica (EnHS), Giardia lamblia (Gial). Some of Aspergillus (As), Blastocystis hominis (BH), etc. are infectious and usually c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6893C12Q1/686C12Q1/04C12N15/11C12R1/785C12R1/66C12R1/725
CPCC12Q1/686C12Q1/6893C12Q1/6895C12Q2600/16C12Q2521/107
Inventor 董晓艳熊永清王洪
Owner 爱科睿特生物医疗科技(南京)有限公司